In the design process of the oil tank foundation, if the deformation is estimated insufficient, the small impact will be caused bystructures splited, the large impact will be triggered serious accidents such as dumping and course the huge economic losses. Many examples show that all sorts of foundation treatment method can resolve successfully uneven settlement problem of the tank. s foundation reinforcement was applied, combined water filling preloading consolidation method, so as to give full play this feature, the gravel pile drainage effect and oil foundation before water filling preloading was used. Thus could reduce the cost of foundation reinforcement and eliminate foundation consolidation settlement. is a kind of rational economic reinforcement method of the tank foundation. Therefore analysis of deformation trait of gravel pile foundation under large-scale oil tank are both theoretical significance and value.Based on the SKIKADA Algeria to large tanks refinery soft vibro-replacement stone column water filling preloading combined with the field test, controlled systemic the deformation of the tank was the main research targets. The large-scale finite element software Plaxis was applied to establish a two-dimensional finite element model for the foundation type. Axisymmetric was introduced in the numerical simulation analysis and calculation. Compared with different length distribution, length, Exchange rate unchanged, the diameter or change of pile spacing, Pile diameter, pile spacing of exchange rate changes, the length of the cushion course of the tank foundation were analyzed on the differential settlement of gravel pile foundation under large-scale oil tanks; the effects of diminishing the differential settlement by Compared with each parameter; Compared with the relation of lateral displacement and differential settlement, the optimal solution of diminishing the differential settlement were evaluated.Finally, the result of Axisymmetric Compared with the field data. At the same time, the effect of strengthening was evaluated; Through the long-term monitoring of deformation of the deep soil and surface soil, a great number of primitive data were obtained. In this paper, the plane inclination and non-plane inclination of the surface and deep soil, and the sway settlement of the round wall are discussed. The settlement of the oil tank foundation is analyzed and evaluated. Main concluded:(1) in the general design, the gravel pile foundation could be simplified by the circle to simulate tank for 2-d numerical computation.(2)Difference settlement of foundation changes with different pile length distribution scope, which, with long pile:0m-0.33D,0.33D-0.39D or 0.33D-0.42D (D-tank diameter) is a minimum;in the center scope of composite foundation, through fixed pile diameter, reduced pile spacing to increase replacement rate, could make the composite foundation in relatively smaller conditions for displacement rate obtained differential settlement;in the center scope of composite foundation, kept exchange rate, pile diameter is more bigger(and the pile spacing, gravel pile foundation is looser) the difference settlement is more smller, With the increasing pile diameter increased, differential settlement of changing is lesser; When mattress level arrangement rate for oil pot floor space 60%, the unequal settlement achieves is smallest;different adjustment methods of differential settlement by adjusting the length discoved, through adjusted the long pile distribution, the rate of displacement could reduce different settlement, but changes the mattress level the effect is not very obvious;the foundation of lateral displacement of negative differential subsidence appears to be one of the most important features of adjustment.(3) Under the different breaker strip module, gravel pile thrust pad gravel layer, And, with the increase of cushion modulus, the amount of gravel pile is smaller, Below distance head of pile in certain distance, there is no settlement difference problem. The breaker strip module’s change punctures the quantity to the head of pile on the influence to be big, the breaker strip module is bigger, on the head of pile punctures the quantity to be smaller;as foundation stiffness becomes smaller and smaller load or replacement rate or the scope of the lateral inflation will become gradually larger, But, no matter how the various factors, the corresponding changes in the maximum lateral inflation is below ground constant depth (in this case depth for 2.5 m),(4) The water gravel pile construction played a role of vibration, eliminated sand soil liquefaction, elimination of foundation soil, improve the intensity, The vibro-replacement stone column and water filling preloading treatment after reinforcement, the bearing capacity of compound foundation 225kPa eigenvalue, allow to design requirements. The plane inclination and non-plane inclination of the surface met the standard value of deformation of foundation, the soft soil can be improved reasonably by the VCS piles combined with preloading, finite element numerical simulation could be applied to help explain the actually-measured data
Acid fracturing as the stimulation of measures to build capacity after several years of development has been applicated widespread in domestic and foreign oilfield. The development got through a process of single process, single-fluid system to a multi-stage process, composite liquid system. In the stimulation process to III dense reservoir such as Jingbian gas field, the cross-linked acid sand carrying acid fracturing technology had shown a good applicability, and then made a challenge to the therotical model. People did a lot of research on reaction mechanism of acid rock and acid fracturing design mathematical model in domestic and abroad, the main idea is to increase consideration, refining assumptions, seeking a more simple solution and so on. The development got through a two-dimensional, quasi three-dimensional, full three-dimensional crack propagation simulation, single-phase, two-phase, mixed phase complex, non-steady state to steady state, continuous medium heat to the process of heat transfer in porous media.’s found a relatively small exothermic reaction into account in acid fracturing temperature field model, the software associated with is relative lack and cause the considerable error in acid fracturing design. However, the flow of cross-linking and rheological properties of acid in shear and the effect of temperature will have significant adverse effects, cross-linked acid formulation (cross-linking agent, breaker concentration) set by breaker and reasonable return time, invariably depends on the ambient temperature. The actual simulation can be seen through the reaction heat for the temperature field has great influence, in turn affect the etching effective distance, and ultimately affect the stimulation effect.This paper analyzes the cross-linked acid sand carrying acid fracturing process in Jingbian gas field, and the shortcomings of the process and the potential of point while affirming its value. Take a further laboratory studies to the acid system, screen the paid agent composition and the amount, analyzes the effects of amount of crosslinking agent to the acid crosslinking time and viscosity and the temperature to the time of cross-linking. Determine acid – kinetic equation of rock at 60℃, acid – activation energy and the acid rock reaction of hydrogen ion effective mass transfer coefficient De using SARⅢacid rock disc reactor.In the base of previous model, add the acid rock reaction releasing heat to the fracture surface energy equation, discrete and give the coupled solution combined with the continuity equation, energy equation of fluid cracking. Take a calculation and simulation to one well in Jingbian using the experiment to obtain the cross-linked Acid Rock Kinetic Parameters and cross-linked acid Jingbian field acid fracturing sand carrying construction parameters, simulation results show that the convergence of the model, reasonable and consistent with the actual situation. After considering the reaction heat, the temperature change still affect the distance of acid.This paper build three-dimensional gap of the effective distance calculation model compound with three-dimensional velocity field model of acid, and gives the lumping method. In according of the simulation to a gas well data in Jingbian field, show the model can simulate and calculate the actual distance accurately, and ’s suitable for further study and application. In base of the model build, and then analysis the effect of construction emission, liquid temperature, the quatitive of surrounding rock, the original temperature gradient, porosity, casing diameter and other factors to shaft and fracture temperature field distribution in a specific time step,and then give some suggestions to the construction according to the simulation results.
Metallothioneins (MTs) are a family of low molecular weight metal-binding proteins, which contain a high proportion of cysteine. In many aquatic species metallothioneins can be inducted by metals, and the degree of MTs induction is correlated with metal concentrations, therefore the level of metals in aquatic environment can be reflected indirectly by the induction of MTs. At present, MTs in marine animals have been recognized as potential biomarker of heavy metals in seawater and sediment. MTs concentrations in marine organisms are also proposed as an important tool to evaluate the risk of metal exposure and assess theof oceans. In the past two decades, many researchers both at home and abroad have applied MTs to marine pollution monitoring, and developed different analysis techniques and extraction methods. Until now, there is no standard method, neither isolation nor quantification in the studies of marine animals. In this study, exposed to seawater containing cadmium was used as a material, the extraction and of the established mercury saturation method and spectrometric method were optimized.For the mercury saturation method, extraction conditions for maximum MT recovery from viscus and gills, expressed as content of sulfhydryl group (-SH) in the crude extract, were optimized by orthogonal design and single-factor design. The optimized homogenization process of viscus was as follows:the buffer concentration was 0.02 mol/L, pH7.8 and m/v was 1/5. MT yield was primarily affected by Tris-HCl buffer concentration. The optimal process obtained for removing impurity was that the homogenate was centrifuged at 20000g at 4℃for 30 min, and then the collected supernatant was heated at 80℃for 10 min, followed by a second centrifugation at 15000g at 4℃for 10 min.The optimized homogenization process of gills was as follows:the buffer concentration was 0.02 mol/L, pH7.8 and m/v was 1/4. MT yield was primarily affected by Tris-HCl buffer pH. The optimal process obtained for removing impurity were that the homogenate was centrifuged at 25000g at 4℃for 20 min, and then the collected supernatant was heated at 80℃for 10 min, followed by a second centrifugation at 15000g at 4℃for 10 min.When the was optimized, gills in R. philippinarum were used as material, the established mercury saturation method was optimized by single-factor design, expressed as the content of Hg in solution. The optimized determination condition was as follows:the 4-fold dilution of supernatant contained Cd-MT was mixed with HgCl2 solution at 50 mg Hg/L in 10% trichloroacetic acid in the same volume. After incubating for 5 min,1.5-fold of 2% bovine hemoglobin was added and rapidly centrifuged at 20000g for 20 min. The relative standard deviation of this optimized method was 4.95%, the recovery of standard addition was 71.08%-78.86%, and the detection limit was 0.24μgHg/L, equivalent to a tissue concentration of 4.47μgMT/g.For the spectrometric method, the conditions of removing impurity and determination condition for MT from viscus and gills, expressed as content of sulfhydryl group (-SH) in the crude extract, were optimized by single-factor design. The optimal process obtained for removing impurity for viscus was as follows:for per 1 ml of the supernatant, 1ml cold (-20℃) absolute ethanol and 80μl of chloroform were added. Subsequently, the samples were centrifuged and the supernatants were added to 3-volume of cold ethanol, stored at-20℃for 1h and centrifuged at 6000g for 10 min. Then, the pellets after centrifuged were washed with 1.74ml absolute ethanol and 260μl Tris-HCl homogenization buffer (containing sucrose), centrifuged again and the pellets were dried by N2(99.99%). The optimal determination condition was Ellman reagent containing 0.5mol/L NaCl. The relative standard deviation of this optimized method was 3.20%, the recovery of standard addition was 90.09%-96.85%, and the detection limit was 9.98μgMT/g.The optimal process obtained for removing impurity for gills was as follows:for per 1 ml of the supernatant, 1ml cold (-20℃) absolute ethanol and 40μl of chloroform were added. Subsequently, the samples were centrifuged and the supernatants were added to 3-volume of cold ethanol, stored at-20℃for 1h and centrifuged at 6000g for 10 min. Then, the pellets after centrifuged were dried by N2(99.99%). The optimal determination condition was that the NaCl was not contained in Ellman reagent. The relative standard deviation of this optimized method was 2.92%, the recovery of standard addition was 91.88%-101.29%, and the detection limit was 6.61μgMT/g.In short, compared with mercury saturation method, spectrometric method is more simple, repeatable, accurate, low-cost, and materials can be obtained easily, and can guarantee the facticity and dependability of the results. Therefore, can be used for the dependable determination of MTs in marine bivalve such as R. philippinarum.
Hyaluronic acid (HA) has been widely used in biomedical fields, because of its good hydrophilicity, viscoelasticity, lubrication, moisture and other properties. However, the price is high, then organization and fermentation production of HA is limited, so HA is far from market demand. Chitosan (CS) is a abundant and cheap natural polysaccharide, its structure and properties are closed to that of HA. Therefore, In this paper, simple technics to prepare Hyaluronic acid likeusing CS was investigated. was prepared water soluble , and the single factor of amount of sodium hydroxide, chloroacetic acid, reaction time and temperature has been studied. Then is found that the product of the degree of substitution greater than 0.6 has a good water soluble, and the product has no skin irritation. Compared with the HA, the product of the degree of substitution greater than 0.75 has preferable moisture retention ability and moisture absorption ability. The chitosan reaction conditions were optimized through orthogonal experiment. The optimum conditions for chitosan were as follows: the mass ratio of sodium hydroxide, chitosan and chloroacetic acid was 4:1:1.4, the reaction temperature was 60℃, the reaction time was 4h, and the degree of substitution of the product was up to 1.186, viscosity is up to 590ml / g, and molecular weight is 6.12×10~5 Da.Then three sample of NOCC E, NOCC B, NOCC G were obtained by ethylene glycol diglycidyl ether, 1,4 butanediol diglycidyl ether and glutaraldehyde crosslinked with carboxymethyl chitosan. Respectively, the viscosity of the three were 2150 ml/g, 2135ml/g and 2295 ml/g, and the molecular weight were 1.708×10~6 Da,1.698×10~6 Da and 1.799×10~6 Da. Both of the aspects were closed to HA.It was found that moisture retention ability and moisture absorption ability of NOCC E, NOCC B, NOCC G was better than HA, and the three samples had antimicrobial activity for S. aureus and E.coli.,whereas HA had no antimicrobial activity, also NOCC E, NOCC B, NOCC G had no skin irritation. Stability research showed that the stabilities of NOCC E, NOCC B, NOCC G were good at bellow 40℃in 48 h. The degradation of three products were both in acidic and alkaline media, and the acidic medium was more obvious. In addition, their stir stabilities exhibited excellent in 48h.Thus the experimental obtained three sample NOCC E, NOCC B and NOCC G, both of which were closed to HA, and moisture retention abilities and moisture absorbtion abilities were better than that of HA. They had no skin irritation and had certain antimicrobial activity.
The silk industry is a traditional industry in China.made important contributions to the economic construction of China. Siklworm as the largest part byproduct of silk industry, the actual utilization of is not available.In this research, the mixed fatty acids was prepared from and the compositions of lipids were analysised by gas chromatography, high performance liquid chromatography ,thin layer chromatography, and other methods. The results show that lipid content of silkworm was about 32.79% on dry basis. The content on lipids was about 70% ,unsaturated fatty acids, theα-linolenic acid content on unsaturated fatty acids was 32.79%.To purify alpha-linolenic acid (ALA) from silkworm lipids, urea crystallization and argentated silica gel chromatogramphy were employed in this work. Results suggested that fatty acid/urea ratio is key factor for ALA purity and recovery, urea concentration, the crystallization temperature and time are the second. When crystallization was carried at 0°C for 8 h, with fatty acids/ urea ratio of 0.1, urea concentrateion of 30%, ALA percentage increased from 30% to 87%. And the enriched lipid sample was subjected to urea crystallization for the second time, the concentration of ALA did not further increased, and the ALA recovery decreased significantly. After the further isolation of ALA from the enriched lipid by using argentated silica gel chromatography (ASGC), ALA percentage increased to 99%, and recovery of ALA was 63%. So the isolation of high purity ALA from silkworm lipids by urea crystallization then ASGC is feasible.
An effective enzyme-producing strain which could hydrolyze gum Arabic was isolated from soil samples of Hangzhou, Shijiazhuang and Huangdao. After further purification, strain named ZHB-306 which enzyme activity reached 25.8 U/mL and enzyme fermentation performance stability was obtained. When growing in the plate, the strain could grow into colonise in 2~3 days. Myceliums were white and dense on the early colonies which protruded flocculent. The color of colonise were from white, pale pink, incarnadine to slightly purple; spores were from light brown to black. The strain is very similar to Fusarium morphologically, the species of which need molecular biology identificatio.Mutation breeding was applied to enhance the ability of producing agarase of the strain ZHB-306 by ultraviolet mutation and the activity of enzymes was increased by 185%.The experiments of single factors and orthogonal design were carried out to optimize the culture medium and cultivation conditions. As a result, the optimal medium composition was as follows: gum Arabic 2.5%, corn flour 0.4%, (NH4)2SO4 0.9%, K2HPO4 0.15%, MgSO4?7H2O 0.05%, KCl 0.05%, FeSO4?7H2O 0.001%, H3BO3 0.001%, pH was adjusted to 6.0. The fermentation for enzymes production was carried out in 250-mL Erlenmeyer flasks containing 30 mL of medium with inoculation of 9% (v/v) seeds, incubated at a agitation speed of 200 r/min and 30℃for about 48 h. The enzymes activity reached 231.6 U/mL under the optimum conditions andwas 368% higher than that before the optimization. Fermentation results could be applied to the further study and industrial production of this enzymes, and the development and application of L-arabinose.The HPLC-ELSD detection method of L-arabinose were established after a series of experiments. Different kinds of sugar could be separated on Hypersil NH2 column with the mobil phase of acetonitrile-water (80:20) at a flow rate of 1 mL/min. The condition of Evaporative light scattering detector were set as followings: the temperature of drift tube was 100℃, nitrogen gas was used as carrier with the flow rate of 2.5 L/min. L-Arabinose could be separated from other saccharides. The correlation coefficient of linear regression equation for L-arabinose was 0.9993. The reproducibility was RSD of 2.03%. The average recovery was 98.7%.The enzymatic conditions of degrading gum rabic into L-arabinose by the enzymes were also studied. The results showed that the optimal time, temperature and agitation rate for enzymatic reaction were 90 min, 50℃and 0r/min respectively. The optimal substrate was 0.3% (w/v) gum arabic which pH4.0. Determination of L-arabinose after digested with the best conditions by using HPLC-ELSD method, the concentration was 530.6 mg / L, and thus calculated L-arabinose yield was 59.8%. Therefore, was essential to do some further researches on enzymological properties of the enzymes.
UV coatings are environmentally friendly coatings. They are widely used on such heat-sensitive substrates as plastic, wood etc., for its pollution-free, fast curing and energy saving properties. The substrates as plastic, wood etc., are claimed for flame resistance in special situation. has hindered its further application. Therefore,is significative to study the flame resistance of UV coatings used in these substrates.A new nitrogen flame retardant is applied to UV coating. By means of horizontal flame test, DSC, FTIR and so on, we studied the effect of POP-290 and different monomers (TMPTA, HDDA, TPGDA and so on) on the UV coatings and the relationship between and the horizontal burning grading. The results showed that:With the increasing of POP-290 content,both and the horizontal burning grading increased. After blending the nitrogen flame retardant with acrylate UV coating, the flame retardance of the UV coating got effectively improved, and the increased from 21 to 27. The POP-290 took part in UV-curable reaction, which improved the compatibility. was found that the gel content of the film cured increased with the increasing of POP-290 content. With the increasing of monomer content, the flame retardance got worse. The DSC showed that POP-290 improved the Tg of EA coating.These results made possible to modify UV-curing material by adding hybrid nitrogen flame retardant. And it provided the great reference to the UV-curing technique.
Study on Enantiomer Separation of Triazole Chiral Fungicides with Supercritical Fluid Chromatography
Many enantiomers of chiralpesticides show different biological activities and toxicity, so the separation of enantiomers is very important. In this paper, the enantioseparation of several typical chiral pesticides(herbicide ) were investigated on two commercial polysaccharide -derivative chiral column using supercritical fluid chromatography (SFC) and high performance liquid chromatography (HPLC).At first, the chiral separation of seven s was performed on Chiralcel OD and Chiralpak IB using SFC and HPLC. The influence of chiral stational phase, temperature, mobile phase additives and its concentration on the enantioseparation was studied. At last, five fungici des such as triadimefon, hexaconazole, uniconazole, myclobutanil, and difenoconazole got baseline separation on Chiralpak IB. On Chiralcel OJ, only tebuconazole and imaizole got baseline separation. Using SFC, the highest Rs values of myclobutanil, triadimefon, hexaconazole, tebuconazol e, uniconazole and difenoconazole on Chiralpak IB were 15.47, 1.28, 5.25, 1.85, 2.28 and 1.06/0.735 respectively, the highest Rs values of myclobutanil, hexaconazole, imaizole, tebuconazole and uniconazole on Chiralcel OJ were 1.02, 0.86, 3.98, 5.8 and 1.42 respectively. Using HPLC, the highest Rs values of myclobutanil and imaizole on Chiralpak IB were 9.11 and 0.75, on Chiralcel OJ were 2.12 and 3.52.Secondly, ethofumesate, diclofop-methyl, acetofenate, and benalaxyl were separated on Chiralpak IB and Chiralcel OJ using SFC and HPLC. The influence of chiral stational phase, temperature, pressure, mobile phase additives and its concentration on the enantioseparation was studied. At last, all the four pesticide were baseline separated. Using SFC, the highest Rs values of acetofenate, benalaxy, ethofumesate and diclofop- methyl on Chiralpak IB were 1.47, 4.68, 3.03 and 4.41 respectively, the highest Rs values of benalaxy and diclofop-methyl on Chiralcel OJ was 3.18 and 1.18. Using HPLC, the highest Rs values of benalaxyl and diclofop-m ethyl on Chiralpak IB were 5.44 and 4.81, on Chiralcel OJ were 1.79 and 1.36.
The Structure Molecular Designation and Antimicrobial Activity of Antibacterial Peptide from Mytilus Coruscus
Antibacterial peptide is a class of small molecular peptides produced by the organism when’s resisting pathogenic microbes. has the advantages of high efficient sterilization and low risk of drug resistance, so it has the broad prospect in developing new organism antibiotics. Now the research on marine s becomes one of the hot topics.We studied the -1 derived from serum of Mytilus coruscus and simulated its spatial structure for studying the relationship between spatial structure and antimicrobial activity and providing new ideas of manual designation and modification of . For verification of the functional fragment of Mytilin-1, we synthesized two decapeptides corresponding to the main mimic structures connected theα-fold andβ-hairpin of mytilin-1 from Mytilus coruscus and evaluated their activities. The two decapeptides, which named MDP-1(Mytilin derived peptide-1) and MDP-2, were re-designed and synthesized by solid-phase peptide synthesis methods.The antibacterial test of MDP-1 and MDP-2 shows that MDP-2 has stronger activity than MDP-1 despite the fact that both peptides can inhibit the growth of Gram-positive, Gram-negative bacteria and fungi. MDP-2 possesses the obvious inhibition to marine vibrio and pathogen. MDP-1 and MDP-2 have strong thermal stability, and their antibacterial activities didn’t drop obviously after culturing in the human plasm for 24h, 37℃.For better understanding the relationship between spatial structure and function of MDP-1 and MDP-2, we analysis their spatial structure by 1H NMR。The result indicates that compared with structure of Mytilin-1, MDP-1 and MDP-2 have no regular structures ofβ-hairpin in loop area, and they have irregular loop structures instead. The amino acid sequences of MDP-1 and MDP-2 are opposite, which makes the trends of main chains are different. Meanwhile, the position of residues in the side chains are different, especially the position of Arg. The two Args of MDP-1 distribute on opposite side of the loop, but the two Args of MDP-2 distribute on same side, which help to make the positive charge more concentrate. According to the previous research, the two Arg are considered the functional part of antimicrobial activity. So the distribution difference of the two Arg may determine the intensity of antimicrobial activity.The results lay a foundation for further research on the antibacterial mechanism of Mytilin and for further exploitation of s with lower molecular mass and more stable structure.
Polymer light-emitting diodes (PLED) have recently attracted a great deal of attention due to their physical properties and their potential applications in light emitting device and flat panel displays. To develop a full-color display, the design and synthesis of light-emitting materials are particularly important. The, ′-spirobifluorene-based polymers are one of the most promising candidate for use in blue PLED because of the special spiro-structure. Polyspirobifluorene is a classic blue-emission polymer and a good electron-transporting material. Previous studies have shown that incorporating a ,9′-spirobifluorene linkage into the structure of polymeric materials, leads to a reduction in crystallization tendency and an increase in glass transition temperature. Also most of the desired electronic and optical properties of the corresponding nonspiro molecules are preserved. However, poor processability due to rigid ring structure is still a problem for preparation and application for devices.In this paper, various wide bandgap conjugated polymers such as polycabazole and polytriphenylamine are introduced into 9,9′-spirobifluorene unit, via , and two series of the polymers are poly(9,9′-spirobifluorene-co-cabazole), poly(9,9′-spirobifluorene-co-triphenylamine). FTIR spectra is used to characterize the polymer structures. The PL spectrum of polymers indicates that there is a wide PL maximum around 410 nm, and the emission color of the polymers is blue. The shoulder peak of Poly(SPF-CAS) in the PL spctrum disappears when the feed ration of SPF/CAS is 1:3.Besides we design and synthesize spirobifluorene-based polymers having alkoxy hydrocarbon chains. As well known, the performance of solubility and electron transport can be further improved when copolymerized with alkoxy side chain moiety by the chemical modifications. The alkoxy side chain group plays an important role in improving the solubility of the corresponding copolymers. So a series of polymers are synthesized via . UV-vis absorption spectra of polymers show that the absportion peaks are a single sharp in diluted solutions. The polymers films spin-coated from chloroform(CHCl3) solution onto a quartz plate are shown that the position of UV-vis absportion peaks red-shifts 6-10 nm compared with the polymers in solutions. The PL spectrum indicates because of increasing the length ofπ-π*conjugate chain, the spectrum of the emission and the excitation overlap which means is easy for the efficient energy to transfer. With quinine sulfate solution as a reference, three of polymers are measured to be 0.7. Thermal analysis results show that the high thermal stabilities of these polymers with the rigid and bulk spiro-segement unit in the polymer main chain. Cyclic voltammetry (CV) tests indicate that the highest occupied molecular orbital (HOMO) energy levels of the copolymers are located between -5.85 and -5.69 eV. The solubility test shows that good solubility can be attributed to the presence of the long alkoxy chain in the polymers. is noteworthy that the polymers present best solubility when the feed of ration of 9,9′-spirobifluorene and alkoxy-benzen is 1:1.