In this paper, the modification of KGM and apply them in urease immobilization have been researched. Concentrate on sponge KGM carrier, cross-linking KGM carrier and KGM-ALG microballoon sphere. Besides these, properties of the free urease and immobilized urease were also discussed. The characterizations by light microscope, SEM and FT-IR provide academic supports.The conduct of low temperature treatment before infusibility treatment made the carrier show an appearance of sponge and springiness. Its’ properties such as merchanic intensity, storage stability and using ability are all improved. And it boasts a high sensitivity in the change of pH and has a better compatibility on substract.The cross-linking immobilized urease enjoys great storage stability, after stored in 4℃for about 30 days, its’ enzyme activity appears litter lose. When we install 10.0g immobilized urease of this kind into a pillar and use it to catalyse urea-phosphate buffered solution for 3 days in run, totally catalyse 1000ml urea-phosphate buffered solution, and then sealed it with film, stored it in 4℃for around four months, and the enzyme activity remains 40%.KGM-ALG microballoon sphere immobilized urease boasts good enzyme activity over 8.9U/g. We can adjust the enzyme release rate and merchanic intensity through changing the proportion of KGM, ALG sol and the concentration of chitosan. The method is easy-conduct, mild-condition, fitting many substances’ immobilization from active protein medicine, enzyme to hydrophobe, and available for massive production.

This dissertation is organized in two parts:Part 1:NMR Studies on the Interaction between Chitosan and 1-HydroxybenzotriazoleThe interaction between Chitosan（CS） and 1-Hydroxybenzotriazole （HOBt） wasstudied by ¹H NMR spectroscopy,NMR diffusion measurement,and solid statehigh-resolution ¹³C NMR spectroscopy.CS and HOBt were found to be able to formorganic complex,and the main binding force is hydrogen-bond interaction.Theassociation constant of CS/HOBt complex was calculated from the self-diffusioncoefficients of HOBt.CS/HOBt system would be efficient in chemical modificationof CS without the use of acids or organic solvents.Part 2:Preparation and NMR Study of CS-g-mPEG/DNA ComplexesWater-soluble Chitosan was prepared by functionalizing with poy（ethyleneglycol） methyl ether（mPEG） at the ammo and hydroxyl groups via a single reaction ina CS/HOBt aqueous system.The product was characterized by NMR,IR etc.TheCS-g-mPEG was complexed with DNA at various CS-g-mPEG/DNA（N/P） chargeratios and the resulting complexes was characterized by IR,gel retardation,dynamiclight scattering,solid state NMR ³¹P DD/MAS spectra and ¹³C CP/MAS spectra todetermine the complex formation and the interaction between CS-g-mPEG and DNA.The results indicated that:The solubility of CS was improved to 50mg/mlH₂Othrough functionizing with mPEG and the ¹H spin-diffusion NMR experiments with¹³C detection showed their good compatibility;the CS-g-mPEG/DNA complex was formed at higher charge ratio（N/P>10）.In the ³¹P DD/MAS spectra,the chemical shiftof the phosphate group of DNA was found to be dependent on the charge ratio.Thesame phenomenon was got in the ¹³C CP/MAS spectra.Both of these results indicatethat higher the charge ratio,then better the compatibility of the system.

Chitosan is the natural polymer containing amido and hydroxyl groups, which can coordinate with metal ions well. Its adsorbing capacity can be enhanced by changing chemical characteristic of the molecule, and its treatment of metal ions containing waste water is paid attention in recent year.Firstly, the schiff’s bases between the benzaldehyde and the chitosan at the normal temperature was studied in this paper, then chitosan was cross-linked with epichlorohydrin and ethylenediamine; protection group drawn off by using hydrochloric acid after cross-linking, finally, chitosan was xanthan acidificated by carbon bisulfide, and the final production was gained at the same time. A best reaction condition was gained through orthogonal experiment: materiel ratio is 1:2, reaction temperature is 40℃, and -OH medium’s chroma is 0.2mol/L,reaction time is 4 hours. And many analyzing measures such as FTIR, XRD and element analysis was adopted for characterizing the CTSN-CS2 and its middle production, the results showed that the product was correspond with the results supposed before.The adsorbent activity for Cu²⁺、Ni²⁺、Pb²⁺ of CTSN-CS2 was studied from five aspect namely, adsorption capacity、medium acidity、adsorption kinetics、adsorption isotherm and regeneration. Tests showed that the adsorption of CTSN-CS2 on Cu²⁺、Ni²⁺、Pb²⁺ were 2.645mmol/g、1.943mmol/g、1.856mmol/g in initial concentration 3.0mmol/L of heavy metal ions solution. The adsorption performance was influenced dramatically by medium acidity. When the pH was low, the absorbent’s adsorption capacity was quite low to three kind of metal ion’s. Along with the pH ‘s elevation, the metal ion’s adsorptive capacity increased. The best pH was 5-6.CTSN-CS2 had the quick dynamics speed to three kind of ion’s adsorptions, was quickest to the Cu²⁺ adsorption, approximately 4h achieves balanced, and the adsorption rate order is KCu> KNi > KPb. The tests of Adsorption kinetics showed:the uptake of CTSN-CS2 Cu²⁺、Ni²⁺、Pb²⁺increase with the increasing of initial concentrations until reache plateau. The uptake datas were analysed by the Langmuir isotherm and the Freundlich isotherm, and show the adsorption isotherms could be well fitted by the Langmuir equation. In the Cu²⁺、Ni²⁺、Pb²⁺ ternary system, the adsorption capacity for Cu²⁺、Ni²⁺、Pb²⁺ of CTSN-CS2 declined. It had the high adsorption capacity and the adsorption selectivity to Cu²⁺, lower to Ni²⁺and the Pb²⁺ adsorption capacity and the adsorption selectivity.

Aryl ethers are important compounds, and there are also important applications in pesticides, pharmaceuticals, fragrances, dyestuffs and so on. Recently, more and more research has been focused on their synthesis. The Pd-catalyzed synthesis of aryl ethers has been reported, however, this catalytic process has many disadvantages, such as the multiple intermediate products, lower yield, difficult recovery of catalyst and high cost. Chitosan is the natural polymer containing -NH₂ and -OH groups, which can coordinate with metal ions well. Since the chitosan-supported noble metal catalysts have high stability, little causticity and low-solvents-solubility, all of which is easy to the separation, recovery and reuse of catalysts. The research and application of the natural polymer chitosan-supported palladium catalyst is paid attention in recent years.First of all, the survey of research on aryl ethers and supported palladium catalysts, and the recent advance in the study on the chitosan-supported palladium catalyst applications were reviewed in this paper. According to the investigation of reference, it shows that the palladium complexes used as catalysts to synthesize aryl ethers had been studied, but the chitosan-supported palladium complex for this catalyzed synthesis has not been reported.Secondly, Natural chitosan-supported palladium, modified chitosan with salicylad- ehyde palladium complex, and SiO₂ supported chitosan Schiff-base palladium catalys- ts were prepared by immersion method using chitosan and palladium chloride as raw material. These catalysts were characterized by FT-TR and NMR; the results showed that chitosan had coordinated with palladium well. At the same time, the synthesis method of 4-nitrodiphenyl ether was discussed. It is concluded that there is a relative reasonable route for this synthesis of 4-nitrodiphenyl: this reaction use p-nitrochlorob- enzene, phenol and sodium hydride as material, toluene as solvent, chitosan-supprted palladium as catalyst.Thirdly, the performance of chitosan-supported palladium catalyzed synthesis of diphenyl ethers was studied. An orthogonal test was designed; Effects of the molar ratio of materials, reaction temperature, reaction time and catalyst consumption on the yields were investigated. The results indicate that the optimal reaction conditions are: 1:1molar ratio of phenol and p-nitrochlorobenzene, 2:1molar ratio of sodium hydride and p-nitrochlorobenzene, 30mL toluene, 0.20g catalyst, 65℃for 9h under an atmos- phere of nitrogen. Under these conditions, the yield of 4-nitrodiphenyl is 79.0%. Similarly, under the optimal reaction conditions: 1.5:1molar ratio of phenol and p-nitrochlorobenzene, 2:1 molar ratio of sodium hydride and p-nitrochlorobenzene, 30 mL toluene, 0.20g catalyst, 100℃for 10h under an atmosphere of nitrogen, the yield of 4-phenoxyaniline is 78.4%. The catalyst has good activity and good repeatability, for it can be separated easily from the product with extraction, then reuse for many times.Finally, the chitosan-palladium complex catalysts on SiO₂ were prepared, and the catalytic performance of this catalyst in the synthesis reaction of alkyl aryl ethers was explored. The research reveals that this catalyst has good catalytic activity in the reac- tion, especially in the synthesis reaction of p-nitroanisole by using p-nitrochlorobenz- ene, methanol and sodium hydroxide as raw material, the reaction condition is also mild.In a word, these catalysts are easily prepared, separated from the reaction mixture, reclaimed and reused, meanwhile, they perform high activity for the synthesis of aryl ether.

The rapid development of modern biotechnology brings about many protein and peptide drugs, which are degradable by proteolytic enzyme in intestinal and not oral. Moreover, most protein and peptide drugs are hard through the biological barrier, resulting in low bioavailability, and only can be used by injection and perfusion. The most drugs release quickly after administrated, which brings the drug level increases rapidly and decreases after reaching the peak. For drugs, the effect is closely related to the concentrations in serum. So the fluctuation of concentration leads to unaccepted side-effect at the peak, and inadequate treating effect at low drug concentrations. Drug delivery system is catering to said problems, and becoming an important developing in drug field recently, so the selecting and researching of drug carriers or delivery materials become a research focus. The delivery materials with extensive sources, poisonless and harmless, and high encapsulation efficiency are pursued by people.In this paper, the delivery material of hydroxypropyl-chitosan was prepared by hydrophilic modification of chitosan, basing on researches for the condition of forming chitosan particles and the effect of drug delivery. Then by coupling folate which has the target effect of tumor to hydroxypropyl-chitosan, the folate-hydroxypropyl-chitosan was prepared and the drug delivery result of folate-hydroxypropyl-chitosan particles was studied. The major contents and results include:1. The chitosan nanoparticle was prepared by ion gel. The condition of chitosan forming sphere was determined and the nanoparticle was characterized by TEM. The results of encapsulation and delivery were studied by taking bovine serum albumin (BSA) as model drug. The results showed that the BSA encapsulation efficiency and the BSA loading were affected by the initial CS concentration and the initial BSA concentration. The higher was the initial chitosan concentration, the higher the BSA encapsulation efficiency and the BSA loading. However, it showed opposite trend when the initial BSA concentration increased. The highest encapsulation efficiency and loading of BSA reached 86% and 49%, respectively. The behavior of chitosan nanoparticles for BSA in vitro release reveals a controlled and continuous release of the entrapped protein after 12 hours and releases 30% of the BSA loading within 2 hours.2. By the reaction of propylene oxide and chitosan under alkaline condition that introducing hydroxypropyl to chitosan, the water-soluble hydroxypropyl-chitosan (HCS) was prepared. The synthesis of hydroxypropyl-chitosan was determined by Fourier Transform Infrared Spectroscope. The hydroxypropyl-chitosan particles were observed by TEM, and the effect of encapsulation and delivery was also researched. The results suggested that the prepared hydroxypropyl-chitosan particle was characterized by regular sphere, uniform distribution of particle size, high encapsulation efficiency, high drug loading and good delivery, and the highest encapsulation efficiency and loading of BSA reached 86% and 46%, respectively. The behavior of HCS nanoparticles releases 28% in average within 2 hours and reveals a continuous release of the entrapped protein.3. The folate-hydroxypropyl-chitosan (FHCS) was prepared by the folate coupled to hydroxypropyl-chitosan under the condition of neutral aqueous solution and dark environment. The effects of temperature, concentrations and time on FHCS yield were studied. The result suggested that the optimal values of content, time and temperature were FHCS 2.0mg/ml, folate 120μg/ml, 70min and 80℃, respectively. The highest yield is 31.3%. Then the folate-hydroxypropyl-chitosan particles was prepared by ion gel, characterized by TEM,and meanwhile, the effect of encapsulation and delivery were researched. The results showed that the prepared folate-hydroxypropyl-chitosan particle had advantages of regular sphere, uniform distribution of particle size. And the highest encapsulation efficiency and loading of BSA reached 90% and 48%, respectively. The FHCS nanoparticles releases 26% in average within 2 hours and reveals a continuous release of the entrapped protein.

Chitosan is a production of the chitin part takes off acetyl-group, chitin is the second amyloses only next to cellulose broad exist in low grade animals such as crab, shrimp and in low grade plants such as alga, fungus. Its contents are extremely rich, nature output every year may amount to 10 tons . It has recently attracted great attention of numerous investigators in different countries because of its unique chemical and biological properties.The first part of this paper dealt with the factors affecting the preparation of 2-Hydroxypropyltrimethyl ammonium chloride chitosan (CTS-ETA). In order to obtain the optimum process conditions, the effects of some factors, such as dosage of etherifying agent, reaction time, reaction temperature or reaction ratio, degree of substitution ,were studied. The chitosan quaternary ammonium salt was characterized by fourier transform infrared spectroscopy (FTIR)Secondly, a copolymer was obtained from graft copolymerization of 2-Hydroxypropyltrimethyl ammonium Chloride Chitosan and acrylamide in nitrogen atmosphere by using ceric ammonium nitrate as redox initiator. It was found that the grafting percentage is 29.5%, viscosity is highter than 57mpas when the concentration of reaction temperature is 35℃, reaction time is 4h. The grafted copolymer was identified by FT-IR.In the third part, the effect of CTS-ETA on the physical properties, aging resistance properties and antibacterial activity of handsheets were studied in this part, the product with different DS and concentration was sprayed on the handsheets or internal added in the pulp respectively. The result showed that the properties of handsheets internal added by DS 1.015 and concentration of 0.2% CTS-ETA changed greatly, the tensile strength increased 15.7 %, the folding endurance increased nearly 54%, and the tearing index and bursting index increased 12.8% and 16.6% respectively. When the handsheets were treated by sprayed the tensile strength, folding endurance, the tearing index and bursting index increased 18.5%,29.7%,15.5%,18.8% respectively. Aging resistance could also be improved by treatment of CTS-ETA, but there is no influence on brightness and gloss.The effect of CTS-ETA on the antibacterial activity of handsheets was investigated. It was showed that the obtained quaternary ammonium salt of chitosan was water soluble and was of good antibacterial effect with low dosage. The handsheets which treated by internal added in the pulp with CTS-ETA (DS=1.015) at a concentration of 0.25% inhibit the growth of Gram negative bacteria ( Escherichia coli) and Gram positive bacteria ( S taphylococcus aureus).The inhibitory ratio to each of them is 63.5%,73.2%. The handsheets which treated by sprayed on the handsheets could inhibit the growth of S. aureus completely at a solution concentration of 0.25% (DS=1.015) and could inhibit 90% of E.coli’s growth. The CTS-ETA definitely has an inhibitory activity against S. aureusa and E. coli.The CTS-ETA-g-AM can be used as paper strengthen agents. The experiments showed that they can improve the physical properties of handsheets made of softwood pulp. adding only 0.3% dosage of the derivates in the pulp the tensile strength, tearing index, bursting index and folding endurance increased 18.1%, 6.9%, 17.1% and 56%, respectively, CTS-ETA-g-AM (viscosity=57mpas) could inhibit the growth of E.coli and S.aureus significantly. The inhibitory ratio reached 29.4% and 57.8% for E. coli and S.aureus at a concentration of 0.3%.

In this paper, the method of the chitosan coating cassava starch/ cellulose basic materials was used to make chitosan coating starch-based composite materials, and the mechanical properties, water transmission, antibacterial properties, compatibility, microstructure of composite materials were studied. The results showed that:1. With increasing of the content of cellulose from 0% to 40%, tensile strength (TS) of cassava starch/ cellulose basic materials increased from 2MPa tol2 MPa, but elongation at break(E) of cassava starch/ cellulose basic materials decreased from 130% to 10%. With increasing of content of glycerol, TS decreased, but E increased.2. Coating chitosan can improve mechanical properties of composite materials. Especially, when the content of cellulose less than 20%, the TS of cassava starch/ cellulose basic materials were 1～3MPa, but TS can increase to 5～7PMa after coating. Comparing improvement of mechanical properties through coating three kinds of chitosan solution which concentrations were 1%, 2%, 3%, these samples which were coated by 2% chitosan solution were the best.3. The effect of water on composite materials was obvious. With water losing, TS of composite materials increased from IMPa to 10MPa, E of composite materials were increased from 22% to 35%, then decreased to 5%.4. Water resistance of no coating composite materials were bad. Dipped in 40℃water 10 minutes, Reserved tensile strength and reserved elongation at break of no coating composite materials were 4%, 19.7% respectively. After the first time coating, reserved tensile strength and reserved elongation at break of composite materials were 16.7%～30.5%, 63.5%～70.3% respectively, After the second time coating, reserved tensile strength and reserved elongation at break of composite materials were 22.9%～46.4%, 82.3%～88.2% respectively. Mechanical properties of materials which were dipped in 50℃water were faster losing than which were dipped in 40℃water.5. The effect of relative humidity (Rh) on mechanical properties of composite materials were obvious. when R_H increased from 36% to 89%, TS of cassava starch/ cellulose basic materials decreased from 4.5MPa to 1MPa, and TS of composite materials decreased from 7MPa to 2MPa. E of composite materials increased first, then decreased. When R_H was 69%, E was the most.6. After the first time coating used chitosan solution which concentrations were 1%, 2%, 3% respectively, only the composite materials which were coated by3% chitosan solution had antibacterial properties. After the second time coating, all materials had antibacterial properties. The order of antibacterial properties of composite materials to four bacteriums was: St.aurous> Escherichia coli> Bacillus subtilis> Penicillium. The rate of antibacterial tests showed that: after 24 hours, The rate of antibacterial of composite materials which were coated two times by chitosan solution which concentrations were3%, 2%, 1%, were 90%, 55%, 45% respectively. After the coating of composite materials were destroyed by machine, antibacterial properties of composite materials would be come worse or lose.7. Scanning electron microscope (SEM) showed that: cassava starch became uniformly continuous phase. Coating changed the faces of the no coating composite materials which apertures, disfigurements were modified. Infrared ray and X-ray of composite materials showed that: coating and basic materials affected each other and had good compatibility.8. Attachment strength test show that: coating can attach basic materials well. After the second time coating chitosan solution which concentrations were 1%, 2%, 3%, the rate of integrity of 2 part of the composite materials were 85%, 95%, 81% respectively. In 99.4℃high temperature steam deposited 1 hour, After the second time coating, the coating which fashioned from chintosan solution which concentration was 3% can prevent composite materials from absorbing water.

In this paper, the method of the chitosan coating cassava starch/ cellulose basic materials was used to make chitosan coating starch-based composite materials, and the mechanical properties, water transmission, antibacterial properties, compatibility, microstructure of composite materials were studied. The results showed that:1. With increasing of the content of cellulose from 0% to 40%, tensile strength (TS) of cassava starch/ cellulose basic materials increased from 2MPa tol2 MPa, but elongation at break(E) of cassava starch/ cellulose basic materials decreased from 130% to 10%. With increasing of content of glycerol, TS decreased, but E increased.2. Coating chitosan can improve mechanical properties of composite materials. Especially, when the content of cellulose less than 20%, the TS of cassava starch/ cellulose basic materials were 1～3MPa, but TS can increase to 5～7PMa after coating. Comparing improvement of mechanical properties through coating three kinds of chitosan solution which concentrations were 1%, 2%, 3%, these samples which were coated by 2% chitosan solution were the best.3. The effect of water on composite materials was obvious. With water losing, TS of composite materials increased from IMPa to 10MPa, E of composite materials were increased from 22% to 35%, then decreased to 5%.4. Water resistance of no coating composite materials were bad. Dipped in 40℃water 10 minutes, Reserved tensile strength and reserved elongation at break of no coating composite materials were 4%, 19.7% respectively. After the first time coating, reserved tensile strength and reserved elongation at break of composite materials were 16.7%～30.5%, 63.5%～70.3% respectively, After the second time coating, reserved tensile strength and reserved elongation at break of composite materials were 22.9%～46.4%, 82.3%～88.2% respectively. Mechanical properties of materials which were dipped in 50℃water were faster losing than which were dipped in 40℃water.5. The effect of relative humidity (Rh) on mechanical properties of composite materials were obvious. when R_H increased from 36% to 89%, TS of cassava starch/ cellulose basic materials decreased from 4.5MPa to 1MPa, and TS of composite materials decreased from 7MPa to 2MPa. E of composite materials increased first, then decreased. When R_H was 69%, E was the most.6. After the first time coating used chitosan solution which concentrations were 1%, 2%, 3% respectively, only the composite materials which were coated by3% chitosan solution had antibacterial properties. After the second time coating, all materials had antibacterial properties. The order of antibacterial properties of composite materials to four bacteriums was: St.aurous> Escherichia coli> Bacillus subtilis> Penicillium. The rate of antibacterial tests showed that: after 24 hours, The rate of antibacterial of composite materials which were coated two times by chitosan solution which concentrations were3%, 2%, 1%, were 90%, 55%, 45% respectively. After the coating of composite materials were destroyed by machine, antibacterial properties of composite materials would be come worse or lose.7. Scanning electron microscope (SEM) showed that: cassava starch became uniformly continuous phase. Coating changed the faces of the no coating composite materials which apertures, disfigurements were modified. Infrared ray and X-ray of composite materials showed that: coating and basic materials affected each other and had good compatibility.8. Attachment strength test show that: coating can attach basic materials well. After the second time coating chitosan solution which concentrations were 1%, 2%, 3%, the rate of integrity of 2 part of the composite materials were 85%, 95%, 81% respectively. In 99.4℃high temperature steam deposited 1 hour, After the second time coating, the coating which fashioned from chintosan solution which concentration was 3% can prevent composite materials from absorbing water.

So many researches indicate that,there are 37 enzymes being able to hydrolysing chitosan non-specifically,such as cellulase、papain、hemicellulase、lipase、lysozyme.Xylanase is one kind of hemicellulase,there is no research report on hydrolysing chitosan specifically.This paper is important meaning in clarifying the mechanism of degradating chitosan of non-specifical enzyme,and is important in enzyme producing low-molecular-weight chitosan with enzyme.This research first had carried on the comparison to the xylanase and other four kinds of enzymes hydrolysing chitosan’s activity.Result indicates:the xylanase is highest active to hydrolysing chitosan. The xylanase’s optimum condition of hydrolysing chitosan is:pH4.75, temperature 60℃,E/S 15%.When the substrate’s viscosity has no influence to the enzyme vigor display, the hydrolysis process of xylanase to chitosan is fit to the Michaelis-Menten equation,and in that,Vmax=15.314μmol/min·L,Km=3.5g/L.That is say:when the substrate’s concentration is enough low,the xylanase is single-substract enzymatic catalysis reaction, and there is only one bind site in a enzyme molecular,and there is only one complex compound in the reaction course.Furthermore,used Sepharose the CL-6B gel chromatography to determine the hydrolysate’s molecular weight distribution,and made a comparison between xylanase’s hydrolysate and other four enzymes hydrolysate’s molecular weight distribution.The result indicates that,hydrolysate’s molecular weight distribution which hydrolysed by xylanase after 6hours concentrates on 8613Da ; hydrolysate’s molecular weight distribution of the inactive enzyme concentrates on 18462Da;after half hour’s hydrolysis,the other four kind enzymes’hydrolate’s molecular weight distribution condition as follows:the cellulase liquid from aspergillus niger on 11943Da,the same as xylanase;the cellulase powder from blue mold on 13317Da;the cellulase powder from aspergillus niger on 12611 Da.Finally,carried on the simple separation purification to the commercial xylanase liquid.In the purification process,the enzyme’s activity has a little loss.The chitosanase activity losed 3000U,xylanase activity losed 5200U.Used SDS-PAGE and HPLC analysing the xylanase’s component,result indicates:the xylanase consists of these molecular weight component 55000 Da,34000 Da,11000 Da;Which one kind or some kind of components has the effect to chitosan hydrolisis, is not clear now,and this needs a further research.

In this dissertation, membranes was preparaed withchitosanCS as main membrane material, glutaraldehyde ( GA ) , tetraethoxysilane ( TEOS ) as cross-linkers, HCl as catalyst. Composite membranes were prepared via interfacial adhesive methed. The composition and structural properties of the membranes were extensively investigated by FT-IR, XRD, TGA , DSC and XPS. The relationships among membrane material, membrane structure and separation performance were discussed.Cross-linked chitosanCS membranes were prepared by in-situ hydrolysis and condensation of tetraethoxysilane (TEOS) within chitosan aqueous solution for pervaporation dehydration of aqueous ethanol solution. The characterization results showed that the introduction of TEOS improved the hydrophilicity and restricted chain mobility of the membranes by formation of cross-linked structure. The remarkable decrease in the degree of swelling was observed with increasing TEOS mass ratio in the membranes and was attributed to the formation of hydrogen and covalent bonds within the membrane matrix. The effect of TEOS mass ratio on the pervaporation of aqueous ethanol solution was investigated. The relationship between the structure of CS-TEOS membranes and their separation characteristics was tentatively discussed. CS-TEOS membranes showed lower swelling degree and improved selectivity for aqueous ethanol solution. Comparing with chitosan control membrane CS-TEOS membrane with 6wt.% TEOS mass ratio exhibited a remarkably improved pervaporation performance with separation factor 460 and flux 281g/m2 h under the identical experimental condition. at 80℃for 90wt.% ethanol aqueous solution.Cross-linked quaternized chitosan membranes were prepared by glutaraldehyde(GA) as cross-linker with various cross-linking densities. The composition and structural properties of the membranes were extensively investigated by FT-IR, XRD, TGA and DSC. The characterization results showed that quaternized chitosan had high hydrophilicity and chain mobility of membranes by formation of cross-linked structure. The remarkable decrease in the degree of swelling was observed with increasing GA content in membranes and was attributed to the formation of covalent bonds in the membrane matrix. The effect of TEOS content on the pervaporation of water/ethanol mixtures was investigated. The membranes with fixed molar ratio 3.9 (GA to molar ratio of 100 repeated unit q-CS) and exhibited the highest permselectivity of 754 with a permeation flux of 756g/m2 h at 80℃for 90wt.% ethanol aqueous solution.Cross-linked CS thin composite membranes were fabricated via interfacial adhesive technique, The results indicated that dispersion of the interfacial adhesive dopamine in the support layer intensified the interface interaction between the cross-linked CS layer and support layer. A homogeneous, defect-free thin film of cross-linked CS on the support layer produced due to a strong interaction between the thin cross-linked CS layer and the support layer. The cross-linked CS separating layer with an approximate thinkness of 4~5μm was formed through dip-coating process. The membranes with fixed mass ratio 3.3 (GA to 100 molar ratio of repeated unit CS) and exhibited the highest permselectivity of 269 with a permeation flux of 1149g/m2 h at 80℃for 90wt.% ethanol aqueous solution. The structural stability of the composite membrane is satisfactory.