The content of this study as follows:This experiment is divided into two steps: the chlorination of glycerol and the cyclization of dichloropropanol.The effect of different factors on chlorination of glycerol was investigated in a packed reactor. On the basis of the Box-Behnken design principle, a quadratic polynomial mathematical model for preparation of dichloropropanol with glycerol was obtained by using the three-level-three-factor response surface methodology, and the influences of the temperature, catalyst loading and flow velocity of glycerol on the yield of dichloropropanol were investigated by determining the yield of dichloropropanol as the response. The optimum condition for preparation of dichloropropanol with glycerol was obtained as follows: the reaction temperature was 106.31℃, the flow velocity of glycerol was 0.6 mL/min and catalyst loading was 7.91 % (w/w). The yield of the dichloropropanol had reached to 88.04 % under the optimal conditions. The influences of the temperature, catalyst loading and flow velocity of glycerol were also optimized with orthogonal design. The results showed that temperature had the largest effect, the second was catalyst loading, and flow velocity of glycerol had the least effect.This step investigated the influence of different factors on cyclization of dichloropropanol. The influences of the mole ratio of dichloropropanol to NaOH,reaction temperature and reaction time on the yield of dichloropropanol were investigated by using the same three-level-three-factor response surface methodology.The experimental results show that the optimum conditions for cyclization of dichloropropanol is as follows: mole ratio of NaOH to dichloropropanol is 1.2:1 and reaction time is 98.15 s at 75℃.The yield of the epichlorohydrin had reached to 88.04 % under the optimal conditions. The orthogonal design results showed that the mole ratio of dichloropropanol to NaOH had the largest effect, the second was reaction time, and reaction temperature had the least effect.The kinetic model of the reaction was established and proved, and the kinetic parameters of the reaction were obtained.The results showed that the cyclization of dichloropropanol is a simple second order reaction, and the activation energies of 1,3-dichloropropanol and 1,2-dichloropropanolare 93.5 kJ/mol and 971.9 kJ/mol.

The condensation of cyclohexanone and glycerol catalyzed by varies catalysts was investigated aiming to study the catalytic mechanism and the effects of catalyst’s structure and property on the conversion of cyclohexanone and the reaction selectivity. The catalytic activities of Lewis acid, proton acid, solid superacid, heteropoly acid, zeolite, montmorillonite and inorganic anhydride were compared. The reaction parameters on the synthesis of menthone glyceryl ketal and cyclohexanone glyceryl ketal were studied and optimized.The result indicates that AlCl3, concentrated sulfuric acid, SO42-/MxOy solid superacid, Hβzeolite, and P2O5 shown higher catalytic activity;and the assayed Lewis acids performed as both Lewis and Bronsted acid. The main factors influencing the catalytic activity of the metallic inorganic salt were: (1) The structure of the Lewis acids: For hydrochloride or sulfate with same anionic moiety, those containing cationic moieties with stronger Lewis acid intensity or larger hydrolysis equilibrium constant performed higher activity and selectivity for 2-methylol-1,4-Dioxaspiro[4,5]decane (a). In addition, the catalytic activity and selectivity of hydrochlorides were higher than that of sulfates if they contain same cationic moieties. (2) The catalytic activity of the hydrated sulfates was significantly influenced by the calcination temperature. The void structure and acidity of solid superacid, zeolite and montmorillonite effect on their catalytic activities, which performed higher selectivity for a, and therefore could be expected to the carriers of other acid catalysts. Product a was favored kinetically, which will transfor to 3-hydroxyl-1,5-Dioxaspiro[5,5]undecane (b) slowly, the later is more stable thermodynamically. With AlCl3 as the catalyst, the reaction mixture obtained after 2 h at the equiblium reaction, and the content of a changed from 97.7% to 94.8% after placing the mixture at room temperature for 60 days.The effect of reactant ratio, reaction time, amount of the AlCl3 and the solvent on the conversion of cyclohexanone and the reaction selectivity was studied, respectively. The prime influence factor was materials ratio. When the molar ratio of the catalyst to reactants was n(AlCl3):n(cyclohexanone):n(glycerol)=1:1000:1500, 10 mL cyclohexane, reaction 92 oC, 1 h, a cyclohexanone conversion of 97.7% and a selectivity for a of 98.0% was achieved, respectively, the product yield was obtained 90.2% (97.4% of purity, GC). AlCl3 can be reused for another 2 runs without obvious deactivity. The reaction conversion was in inverse proportion to the steric hindrance in acetalization.The condensation of menthone and glycerol possible only generate 1,3-dioxolane according to the GC-MS proof. When the molar ratio of catalyst to reactants was n(AlCl3): n(menthone):n(glycerol)=1:100:150, 10 mL cyclohexane, 2 h, a menthone conversion of 43.6% was obtained; and the product yield was obtained as 35.1% (99.1% of purity, GC).

1,3-propanediol is acknowledged as one of six new petrochemical products currently in the world. Its main function is as an important monomer to synthesize a new type of polyester,polyether,polyurethane. 1,3-Propanediol is produced by two ways:chemical synthesis and microbial conversion. Producing 1,3-propanediol by microbial fermentation have many obvious advantages and become the focuses of research.Klebsiella sp. was used as the original strain for further research. A high 1,3-Propanediol-producing strain was obtained by UV and (0.5mg/mL)NTG-ultrasound (200W, 50kHz, 20min) inducing mutagenesis. In batch fermentation of mutant,the production of 1,3- propanediol was increased to 23.31 g/L (by 36.72%). The mutant strain showed genetic stability after 10 generations. The by-product, acetate, was increased, and ethanol was decreased, respectively. The amount of enzyme was not found changed significantly, but the activity of GDHt rises by 21% and the activity of PDOR also rise in a certain extent.The fermentation condition for Klebsiella sp. mutant producing 1,3-propanediol was preliminary optimized by single factor experiment. Different concentration of glycerol, yeast extract, KH2PO4 and other factors influencing the glycerol conversing to 1,3-propanediol was studied respectively. A further study on the fermentation medium composition was by orthogonal test. The optimum medium for mutant was as follows: glycerol 60g/L, yeast extract 6g/L, KH2PO4 3g/L、MgSO4·7H2O 1g/L. In flask fermentation, the production of 1,3- propanediol was increased to 27.03 g/L(by 9.92%).Effects on 1,3-propanediol production of Klebsiella sp. mutant by addition of reductant(VC, GSH, Cys, DTT)were studied.The yield of 1,3-propanediol can reach 29.77 g/L, 29.32 g/L, 29.87 g/L by adding 0.35 mmol/L DTT, 0.35 mmol/L Cys, 0.45mmol/L GSH singlely. They were all exceeded the value of control(27.01 g/L). The yield of 1,3-propanediol is 30.01 g/L(rise by 11.11%)by adding 0.25 mmol/L VC. Studying on the concentration variation of side products by adding VC, the results showed that the final concention of acetic acid, ethanol is increased by 3.77%, 3.35% accordingly. Experimental results also showed that addition of VC had an inhibitory effect on cell growth, but increased 1,3-propanediol production per cell considerably.Effects on 1,3-propanediol production of Klebsiella sp. by addition of ATP were studied. The result showed that addition of ATP had an inhibitory effect on cell growth. Adding 0.04 g/L ATP had a remarkable inhibitory effect on cell growth, the value of OD600 decreased by 26.5%. The yield of 1,3-propanediol is 30.05 g/L(rise by 11.26%)by adding 0.06 g/L ATP. Addition of ATP increased 1,3-propanediol production per cell and the conversion of side product acetate.Comparing to the control, the yield of acetic acid rised by 8.7% by adding 0.06 g/L ATP. Adding 0.06 g/L ATP to flasks at different fermentation stage(0h、4h、18h), the highest yield of 1,3-propanediol is 30.13 g/L(by 11.26%) by adding 0.06 g/L ATP at 4h. The value of 1,3-PD/OD is maximum (rise by 25.84%)by adding 0.06 g/L ATP at 0h, respectively.

Phase change material is able to store thermal energy and release heat promptly, and is therefore contributing to solving the unbalance of energy supply and demand with time and space. In this sense, it may achieve the goals of reducing energy consumption and improving energy efficiency. Phase change materials are currently considered to be suitable for application to the building envelope, through which it could absorb and store heat that enter the room during the day and release it at night to keep the room temperature in a comfortable range thereby lowering heating and cooling energy consumptions of the building.In this paper, a critical review of literature concerning PCMs is presented at first. Melting point and latent heat of PCMs are inherent properties of a material and determined by their molecular or atomic structures, and therefore the key factors associated with the melting point and latent heat of PCMs referring to the crystal chemical bond theory is discussed.According to the selection principle of PCMs, biodiesel byproduct-glycerol, a cheaper resource with low temperature phase change temperature is used. For better performance of PCMs, the mixture of glycerol and 1, 6-hexandiol is recommended. Key performances, including phase change temperature, super-cooling degree, transition heat and thermal stability of PCMs are investigated in experimental apparatus. Referring to phase change temperature, the mixtures are obtained, whose temperatures are between 21 to 28℃.The latent heat of mixtures is smaller than that of the average of polyol. The mixture of latent heat is about 76.4% of the polyol. The maximum deviations of the freezing point and the latent heat of the mixture are 4.6% and 7.8%, respectively, through 100 times cooling and heating cycles.Solid-liquid phase change materials are usually subject to fluidity limit, physical method to absorb PCMs to activated carbon materials is therefore investigated. Through DSC, SEM, TG testing, results show that the 40% content of mixed alcohol is the most appropriate in the form-stable phase change materials.

Dichlorohydrin(DCH) is an intermediate for produce epichlorohydrin. The traditional methods include allyl chloride route and allyl acetate route. The energy consumption and sullage produced in the former method are excessive. In the latter method, flow technics and equipments are complex and costly.Glycerol, as a byproduct of biodiesel, is increasingly available. Under the atmospheric or higher pressure, glycerol can be chlorinated with hydrogen chloride or hydrochloric acid to synthesize DCH, especially 1,3-DCH, by adopting catalysts such as carboxylic acids, carboxylic acids anhydride, esters of organic acids, nitriles and so on. The cost, energy consumption and contamination in the glycerol route is lower than the traditional methods. This technique has been used in the modern industry.In the experiments, the nature of products can be determined depending on gas chromatography analysis associated with mass spectrometry. Internal standard method can be carried out for the quantitative analysis of products and the relative error is less than 2%.In the experiments, catalysts such as carboxylic acids A , B , C,D, E , F and acetonitrile,ZnCl2, SnCl2 etc., are studied. ZnCl2 and SnCl2 have scarcely any effects on the reaction. A acid, B acid and C acid affect the process markedly. But they are prone to be stripped to the collected solution due to their lower boiling points. E acid and F acid have not enough catalysis for application although their losses are lower. Acetonitrile brings on white thick solution containing much solid which is difficult to dispose. D acid has not only enough catalysis, but also can be left largely in the reactor due to its higher boiling point. So, D acid is a perfect catalyst.In the experiments design of orthogonal array by using D acid as catalyst, the better parameters for the reaction are found. Yields of total DCH can reach 88.74%. But the quantity of HCl flow and losses of catalyst are excessive.Based on the experiments design of orthogonal array, the parameters of the optimized experiment are controlled according to the different phases of the reaction. The oil outside reactor is controlled gradually from low to high temperature. Accordingly, the quantity of HCl flow is reduced. Yields of total DCH can reach 91.16%. What is more important is that D acid losses are reduced and the color of products is pellucid. In addition, the losses of material and energy are less than the experiments design of orthogonal array.

The condensation of cyclohexanone with glycerol catalyzed by varies solid acid catalysts was investigated aiming to study the effects of catalyst’s structure and the acid property on the conversion of cyclohexanone and the reaction selectivity. The reaction parameters of the synthesis cyclohexanone glyceryl ketal were optimized. In order to further improve the catalytic activity and investigate the influence of catalyst acidic properties on the catalytic activity, acid-treated montmorillonite and molecular sieve Hβwere modified by loading Lewis acid and protonic acid treatment. Finally, ab initio HF method with 6-31G* basis set level was employed to preliminary study the mechanism of the reaction catalyzed by acid.The result indicates that Hβmolecular sieve are the excellent catalysts for this reaction. Acid-montmorillonite showed better catalytic activity than HY and HZSM-5 molecular sieve because of its mesopore structure. Solid superacid SO₄^2-/Fe₂O₃ and H₂SO₄/C also showed good catalytic activity. Acidification treatment of activated carbon increased its acid content from 0.16mmol/g to 1.83 mmol/g, therefore its catalytic activity was improved. The highest conversion of cyclohexanone and the selectivity of 1,4-dioxaspiro[4,5]decane-2-menthanol reached 99.5% and 98.5% in 1h, respectively, by using Hβmolecular sieve as the catalyst under the optimum reaction conditions: 0.02g Hβmolecular sieve, 0.1mol cyclohexanone, 0.15 mol glycerol, 10ml cyclohexane under refluxing; and the optimum calcination temperature of Hβmolecular sieve was 500oC.As montmorillonite modified by loading FeCl3 and AlCl3, the conversion of cyclohexanone were improved from 69.8% to 77.8% and 74.7%, respectively. This is because the acid content of the catalysts were increased obviously. But the negative result was obtained when using montmorillonite modified by loading ZnCl2 as the catalyst, probably because the reducing specific surface area of the catalyst. The optimum crop load of FeCl3 was 1mmol/g.The conversion of cyclohexanone catalysised by molecular sieve Hβmodified by FeCl3 was improved from 94.6% to 94.9%, but the catalytic activity were decreased when it modified by protonic acid. Thus we hypothesize that the B acid sites and the strong acid site are favorable to this reaction.Quantum chemical method was employed to preliminary study the mechanism of acid catalyzed the reaction. The transition state of the reaction between glycerol and cyclohexanone carbocations was acquired. By using frequency analysis and inner reaction coordinate analysis the rationality of this transition state was affirmed. And the energy barrier of this reaction using protonic acid and AlCl3 are 120.016kJ/mol and 164.774kJ/mol, respectively, and the energy difference is 44.757kJ/mol. Thus, protonic acid has better catalytic activity.

The condensation of cyclohexanone with glycerol catalyzed by varies solid acid catalysts was investigated aiming to study the effects of catalyst’s structure and the acid property on the conversion of cyclohexanone and the reaction selectivity. The reaction parameters of the synthesis cyclohexanone glyceryl ketal were optimized. In order to further improve the catalytic activity and investigate the influence of catalyst acidic properties on the catalytic activity, acid-treated montmorillonite and molecular sieve Hβwere modified by loading Lewis acid and protonic acid treatment. Finally, ab initio HF method with 6-31G* basis set level was employed to preliminary study the mechanism of the reaction catalyzed by acid.The result indicates that Hβmolecular sieve are the excellent catalysts for this reaction. Acid-montmorillonite showed better catalytic activity than HY and HZSM-5 molecular sieve because of its mesopore structure. Solid superacid SO₄^2-/Fe₂O₃ and H₂SO₄/C also showed good catalytic activity. Acidification treatment of activated carbon increased its acid content from 0.16mmol/g to 1.83 mmol/g, therefore its catalytic activity was improved. The highest conversion of cyclohexanone and the selectivity of 1,4-dioxaspiro[4,5]decane-2-menthanol reached 99.5% and 98.5% in 1h, respectively, by using Hβmolecular sieve as the catalyst under the optimum reaction conditions: 0.02g Hβmolecular sieve, 0.1mol cyclohexanone, 0.15 mol glycerol, 10ml cyclohexane under refluxing; and the optimum calcination temperature of Hβmolecular sieve was 500oC.As montmorillonite modified by loading FeCl3 and AlCl3, the conversion of cyclohexanone were improved from 69.8% to 77.8% and 74.7%, respectively. This is because the acid content of the catalysts were increased obviously. But the negative result was obtained when using montmorillonite modified by loading ZnCl2 as the catalyst, probably because the reducing specific surface area of the catalyst. The optimum crop load of FeCl3 was 1mmol/g.The conversion of cyclohexanone catalysised by molecular sieve Hβmodified by FeCl3 was improved from 94.6% to 94.9%, but the catalytic activity were decreased when it modified by protonic acid. Thus we hypothesize that the B acid sites and the strong acid site are favorable to this reaction.Quantum chemical method was employed to preliminary study the mechanism of acid catalyzed the reaction. The transition state of the reaction between glycerol and cyclohexanone carbocations was acquired. By using frequency analysis and inner reaction coordinate analysis the rationality of this transition state was affirmed. And the energy barrier of this reaction using protonic acid and AlCl3 are 120.016kJ/mol and 164.774kJ/mol, respectively, and the energy difference is 44.757kJ/mol. Thus, protonic acid has better catalytic activity.

A new type and nontoxic nonionic surfactant was synthesized and characterized.This new nonionic surfactant can replace ethoxylates which from oil by cheap glycerol. And this production would not decompose to dioxane as ethoxylates,so it was significant to protect environment.This new type green nonionic surfactant was synthesized by glycerol.This expriment was divided into three steps:the synthesize of AGE,the synthesize of ethers of polyglycerol,the performance of ethers of polyglycerol.1. First, synthesized the AGE as a middle-production. This step investigated the influence of different factors on conversion of C12H25OH.The result of orthogonal design showed that mole ratio(NaOH/C12H25OH)had the largest effect, the second was reaction time, mole ratio(ECH/C12H25OH)had the third effect,and reaction temperature had the least effect. A quadratic polynomial mathematical model for preparation of AGE was obtained by using the three-level-three-factor response surface methodology.The optimum condition for preparation of AGE by C12H25OH was obtained as follows: the reaction time was 5 hours,the mole ratio(ECH/C12H25OH) was 1.5,the mole ratio (NaOH/ C12H25OH) was 1.3.The conversion of the C12H25OH had reached to more than 92% under the optimal conditions.2. Synthesized the C12H25OH by AGE and glycerol.This experiment investigated the influence of different factors on yield of ethers of polyglycerol . The results of orthogonal design showed that reaction temperature had the largest effect, the second was reaction time, mole ratio(glycerol/AGE)had the third effect,and catalyst loading had the least effect. A quadratic polynomial mathematical model for preparation of thers of polyglycerol was obtained by using the three-level-three-factor response surface methodology.The optimum condition for preparation of ethers of polyglycerol by glycerol was obtained as follows: the reaction temperature was 150℃, the reaction time was 3 hours,the mole ratio (glycerol/AGE) was 1.15. The yield of the ethers of polyglycerol had reached to more than 83% under the optimal conditions. Some homotypical productions were synthesized from polyglycerols which contain different number of glycerol units.3. The performance of ethers of polyglycerol had been tested in this step.They had good performace in foam、wetting time、emulsification and surface active.

Fuel ethanol, as a clean energy to replace fossil fuels, has become a hot-spot for researchers worldwide. A major problem about ethanol production by anaerobic fermentation of Saccharomyces cerevisiae is that 4 % to 10 % of the carbon source may be converted to glycerol. Strains impaired glycerol synthesis would lead to increase yield of ethanol and efficiency of utilization of the carbon source. The majorst rategy is to interdict or manipulate the metabolism, or to express another gene in S.cerevisiae to redirect the carbon flux. With glucose as the carbon source, glycerol is formed by conversion of dihydroxyacetone phosphate to glycerol-3-phosphate (G-3-P) and subsequent dephosphorylation of G-3-P to yield glycerol. The first reaction is catalyzed by glycerol-3-phosphate dehydrogenase (GPD). There are two isogenes encoding different forms of GPD, GPD1 and GPD2.Firstly, we choose the McClary medium which reached a maximum sporulation rate for Saccharomyces cerevisiae of optimum sporulation medium. Six haploid strains were got after sporulation of Saccharomyces cerevisiae. Take the selected 6 strains and W303-1A as hybridization strains, and make group hybridization, we found two different haploid strains(a type andαtype).Based on homologous recombination, a recombinant plasmid pUC19-GPD1::?Km was constructed and introduced into haploid (a type) by electroporation after linearized. The haploid mutant S.cerevisiae Y(a,△gpd1) was generated and comfirmed by the method of PCR. The recombinant plasmid pPIC-GPD2-bgl-hyg was digested by HindIII and transformed into tne two different haploid strains(a type andαtype)by electroporation, then the haploid mutants S.cerevisiae-Y(a,△gpd2) and S.cerevisiae-Y(α,△gpd2)were generated. Yeast-two hybrid technique used for reference, after hybridization between S.cerevisiae-Y(a,△gpd1) and S.cerevisiae-Y (α) , S.cerevisiae-Y ( a,△gpd2 ) and S.cerevisiae-Y(α,△gpd2), the diploid mutants S.cerevisiae-Y(△gpd1,△gpd1) and S.cerevisiae-Y(△gpd2,△gpd2)were generated and comfirmed by the method of sporulation and PCR.In ethanol fermentation of glucose carbon source, the recombinants S.cerevisiae-Y(a,△gpd1) and S.cerevisiae-Y(α,△gpd2)’s glycerol production decreased by 24.83% and 22.35% than their parent strains. And the recombinants both showed a slight increase in ethanol formation. The result of anaerobic fermentation showed that the rate of growth and glucose consumption of the S.cerevisiae-Y(△gpd1,△gpd1) and S.cerevisiae-Y(△gpd2,△gpd2)recombinants were slower than S.cerevisiae-Y. On the other hand, when compared to the original strain,there were 11.66 % and 20.71 % reduction in glycerol formation for S.cerevisiae-Y(△gpd1,△gpd1) and S.cerevisiae-Y(△gpd2,△gpd2),respectively. Ethanol production increased by 5.9 % for S.cerevisiae-Y(△gpd1,△gpd1) and 13.2 % for S.cerevisiae-Y(△gpd2,△gpd2). Dramatic reduction in acetate and pyruvic acid was also observed in both recombinants compared to the original strains.

1, 3-Propanediol has numerous applications in the manufacture of polymers, foods, lubricants, and medicines. Industrial 1, 3-propanediol production has attracted attention as an important monomer to synthesize a new type of polyester, polytrimethylene terephthalate. 1, 3-propanediol is produced by two methods: chemical synthesis and microbial conversion. However, traditional chemical conversion of 1, 3-propanediol is difficult, which has a low selectivity and consequently its high price hinders the utilization of 1, 3-propanediol in polymerindustries. Interest has now been focused on the production of 1, 3-propanediol by recombinant strain fermentation.The lack of NADH in the glycerol metabolism inhibit the activity of 1, 3-propanediol oxidoreductase, and induce the accumulation of 3-hydroxypropionaldehyde, which can inhibit the activity of glycerol dehydratase, and prevent the cell growth and the production of 1, 3- PD. To resolve the problem, this research was to construct a recombinant strain which could convert glycerol to 1, 3-propanediol. The structure gene yqhD from a wild-type Escherichia coli encoding 1, 3-propanediol oxidoreductase isoenzyme and the structure gene dhaB from K. pneumoniae encoding glycerol dehydratase were amplified by using PCR method. The expression vector pEtac harboring yqhD gene and dhaB gene was transformed into E. coli JM109 to yield the recombinant strain E. coli JM109 (pEtac-dhaB-tac-yqhD). Fermentation experiments were performed by E. coli JM109(pEtac), E. coli JM109(pEtac-dhaB-tac-yqhD) showed that no 1, 3-propanediol was produced in E. coli JM109(pEtac), only the strain harboring both dhaB gene and yqhD gene converted glycerol to 1, 3-propanediol after induction by IPTG. The recombinant enzyme activity analysis showed that the specific enzymatic activity of glycerol dehydratase and 1, 3-propanediol oxidoreductase isoenzyme in E. coli JM109 (pEtac-dhaB-tac-yqhD) were 15 U/mg protein and 10 U/mg protein, respectively. While, the specific enzymatic activity of 1, 3-propanediol oxidoreductase in E. coli JM109(pEtac) were only 4 U/mg protein.While, the recombinant plasmid pEtac-dhaB-tac-yqhD harboring the genes encoding DHAB and YQHD was transformed into E. coli JM109. A stable two plasmid system was obtained via cotransformation of pEtac-dhaB-tac-yqhD and pUC-tac-dhaT into E. coli JM109, which convert glycerol by using two coenzyme (NADH, NADPH), and decrease the accumulation of 3-hydroxypropionaldehyde. And the specific enzymatic activity of glycerol dehydratase and 1, 3-propanediol oxidoreductase isoenzyme in E. coli JM109(pEtac-dhaB-tac -yqhD, pUC-tac-dhaT) were 23 U/mg protein and 12 U/mg protein, respectively. 1, 3-propanediol oxidoreductase isoenzyme activity of E. coli JM109 (pEtac-dhaB-tac-yqhD, pUC-tac-dhaT) was higher to that of E. coli JM109(pEtac-dhaB-tac-yqhD). The production of 1, 3-propanediol on 50 g/L glycerol by E. coli JM109( pEtac-dhaB-tac -yqhD) was 1.52 g/L and the production of 1, 3-propanediol by E. coli JM109(pEtac-dhaB-tac -yqhD, pUC-tac-dhaT) was 1.86 g/L after induction. Meanwhile, the fermentation result showed the increase of 22.3% of 1, 3-propanediol yield by E. coli JM109(pEtac-dhaB-tac- yqhD, pUC-tac-dhaT) was obtained compared with E. coli JM109(pEtac-dhaB-tac-yqhD). The orthogonal design method was then applied to further optimize the fermentation condition of the recombinant strain. A mathematical model was then developed to show the effect of each medium compositions and their interactions on the production of 1, 3-propanediol by recombinant strain E. coli JM109. The model estimated that, a maximal yield of 1, 3-propanediol could be obtained when the concentrations of glycerol, Vitamin B12, KH2PO4, Mg2+ and yeast extract were set at 20 g/L, 0.01 g/L, 7.5 g/L, 0.67 g/L and 5 g/L, respectively. The yield under the optimal parameters and process can reach 2.25 g/L.