The mine exploit, industry wastewater, garbage treatment and other human activity have a serious affection on our country’s under water. So, it is a critical time todevelop a technology of low cost and high treatment effection. A study of Ag⁺、Co²⁺、Sr²⁺、Cs⁺ five ions adsorpted by S. cerevisiae on huge volume and low ions consistency wastewater which has been polluted by heavy metals. Creative points of the study are as follows:1、The experimental results indicated that, Cu²⁺ and Ag⁺ both uptake took place at the best pH range was 4-5; the best biosorption temperature of Cu was 20℃-30℃, temperature had no effect on the progress of Ag⁺ biosorption; there were two progresses of Cu²⁺ and Ag⁺ adsorption, the first was fast and it happened on the cell face mainly was physics adsorption and ion exchange, the second was slow, heavy metal enter cell through initiativr transport and consume metabolism energy. The capacity of Cu²⁺ almost 93.38% uptake was completed within the first ten minutes and the one of Ag⁺ almost was 86.6%; both ions biosorption time was 3 hours.2、The Langmuir and Freundlich adsorption isotherms can well described the progress biosorption of Cu²⁺, but the Langmuir adsorption isotherm was best, it’s related coefficient was 0.999 42 and the adsorption equation was: q =7.928 33·c/（c +1/0.986 7）; the isoadsorption of Ag⁺ accorded Langmuir isotherms, and Fredulich isotherm wasn’t suit, the adsorption equation was: q = 49.7265·c/（c +1/0.00825） and its related coefficient was 0.98572.3、The adsorption ability of Sacchromyces cerevisiae on heavy metals was Sr²⁺>Co²⁺>Cs⁺ through comparation two of them mixed solitio, this maybe related with their moving morphology and the morphology of Cs⁺was simple ion Cs⁺、the one of Co²⁺ was mainly CoHCO₃⁺ and C₀²⁺·xH₂O、the one of Sr²⁺ was mainly SrHCO₃⁺and Sr²⁺·6H₂O.

PM₁₀ and radioactive radon gas are the main pollutants in Uranium ore mining activities. The pollutants of PM₁₀ including radionuclides, heavy metals and organic pollutants and so on. These airborne particles contaminated the environment through the settlement and Air-proliferation.they （especially the diameter of less than 10μm of respirable particulate matter）are easily inhaled in the human body so as to have a serious impact on health.PM₁₀ samples were taken at Uranium mining area of east China in july of year 2007,and analyzed for Uranium、thorium、heavy metal and PAHs by ICP-MS、ICP-AES and High Performance Lipuid Chromatography（HPLC）. The basic characteristics of PM10 pollution were studied, And investigate the thorium and uranium enrichment characteristics of the heavy metals in the moss plants. Followed is the main conclusion that thesis has got:（1） The differences of PM10 concentrations are very large in different spatial distribution. The concentration of particles is 60～80μg/m³ in sampling points 1, 2 and,and 130μg/m³、200μg/m³ in points 4 and 5.The main factors is single source of particulate matter, mainly from TSP of uranium mining and natural particulate matter in sampling points 1, 2 and 3. The source of particulate matter is more complicated in the points 4 and 5, not only uranium mining of the particles brought about long-range transport of pollution, the major is subject to the impact of human life.（2） The chemical elements of PM₁₀ is similar, the main element is composed of inorganic Al, Ca, Mg, which accounting for 75 percent of the sum elements mass, they are rich in the crust of the elements, mainly from the mine dust. In the high concentration of PM10, each element content accordingly higher, but the percentage of PM10 are not necessarily high quality.（3） Through enrichment factor analyzed, wo know that the enrichment factor of Cr, Cu, Pb, Ni,and Zn are more than 10,they are obvious enrich in the respirable particulate matter （PM10） and are the main the inorganic elements of PM10 Pollution, which may be caused by the coupling of participation of ground ore dust and dust （4） The highest concentration of uranium was 3 sampling points,but the concentr-ation of particulate matter （PM10） only 72.61μg/m3, the maximum concentration of thorium was 1 sampling point, and the concentration of particulate matter （PM10） only 70.02μg/m3. The highest PM10 was the sampling points 4 and 5, but content of thorium and uranium are relatively low. It is thought that uranium andthorium are from uranium mining, and the source of particulate matter is more complicated.（5） The heavy metal content of value the moss which in same environment was compared, the different habitats moss plants is not the same of the enrichment of heavy metals,tree>stone>soil.Bryophytes in nutrition mainly from the precipitation and atmospheric particles in the settlement, but the matrix can also affect the moss on the sensitivity of pollutants and enrichment capability, bryophytes matrix for the growth of certain elements of the absorption and assimilation.（6） Through the enrichment capacity of the moss can know that the Entodon compressus C. Mutll on the enrichment of uranium is relatively high, Haplocladium microphyllum, Hypnum plumaeforme the second level of enrichment, Plagiomnium Cuspidatum T. kop on uranium Enrichment is not too good. Haplocladium microphyllum on the thorium higher degree of enrichment, Entodon compressus C. Mutll, Sematophyllaceae Pylaisiadelpha yokohamae general enrichment, Plagiomnium Cuspidatum T. kop enrichment performing poorly. In different sampling points, with a moss on the uranium, thorium not the same degree of enrichment, with the main points of the pollutant concentration and the surrounding environment. The whole of Entodon compressus C. Mutll, Haplocladium microphyllum on the absorption of uranium thorium are strong.（7） The individual PAHs are below 100ng/m³, with the lowest concentration occurring at anthracene（0.21ng/m ） and the highest at benzo[b]fluoranthene（2.31ng/m³）.The overall average concentrations of fluoranthene, chrysene,benzo[b]fluoranthene, benzo[k]fluoranthene, imdeno[1, 2, 3-cd]pyrene, benzo[ghi]perylene are higher, and those of phenanthrene, pyrene, benzo[a]anthracene, benzo[a]pyrene are in the middle, and those of acenaphthylene, acenaphthene, fluorine, anthracene, dibenzo[a, h]anthracene are comparatively lower. The pattern of higher abundance of four and more aromatic rings PAHs in the samples is an indicat or of incomplete combustion process of fossil and fuel at high temperature.

With the rapid industrial development, urban pollution and the increase of agricultural chemicals types, the increasing number of soil heavy metal pollution is becoming increasingly serious. Heavy metal pollutants in soil with poor mobility, stranded in a long time and not be microbial degradation, toxic and cumulative effect of strong characteristics of the crop growth, yield and quality have a greater impact, and through the food chain affect human health, soil Once the subject of heavy metal pollution, is to eliminate an extremely easy. Therefore, investigation and evaluation of heavy metals in soil environment pollution and its risk profile, find out the soil quality of the environment, strengthen the comprehensive control of soil pollution, protection of human health is of great practical significance.Eight kinds of heavy metals (Hg, As, Cu, Pb, Zn, Cr, Ni and Cd) in the black soil region (Dehui)in Jilin receive the sampling for survey and test analysis in this paper. With the aid of Global Positioning System, Geographical Information System and evaluation model integration technology of soil heavy metal pollution, following the national soil environmental quality standard and the value of the local soil background for the evaluation criteria, the single pollution index was used to elevate the pollution degree of single element, and comprehensive pollution index was used to implement the general evaluation of soil environmental quality. And then the single evaluation maps and the comprehensive evaluation map was obtained by GIS technology respectively. Studies have shown that the use of geographic information systems black soil area in Jilin Province of heavy metal pollution assessment, the evaluation method is feasible, visual effects and strong. This paper tries to use two standard evaluation to make up for the evaluation of the possible lack of previous studies and this is the difference.Base on the research results, the spatial distribution low of heavy metal content of soil in study region was revealed, providing scientific basis to the governance of heavy metal pollution and the sustainable utilization of the black soil.

Nong’an County, as the key basic County of the State, not only produces agricultural products for local residents, but also supplies and processes the mainly agricultural production for its capital city, Changchun, and it also supplies millions of tons of marketable grains for our Country. In recent years, with the increasing of population and developing of economy, the heavy metal pollution problem in farmland soils in Nong’an County has been increasingly serious. Researching on heavy metal contamination in farmland soils will play a very important role in protecting more effectively and utilizing rationally the existing land resources, speeding up agricultural restructuring, developing pollution-free agricultural production to achieve standardization of agricultural production, and guaranteeing food and grocery security.Based on the environmental science principles, geography principles, and SPSS technology, geographic information system (GIS), taking farmland soils in Nong’an County as study area, the author evaluated roundly the heavy metal contamination in farmland soils, and disscussed deeply the spatial variation of heavy metals and impact mechanism. The following conclusions were drawn from the analysis.Ⅰ.The quality of the most soil environment was good in Nong’an County. In the 162 soil samples, the single pollution indexes, single-factor composite indexes and multi-factor integrated indexes of Pb, As, Cd, Cr all reached NY/P391-2000 standard. As for Hg, the single index is that 107 samples met NY/P391-2000 standard, 157 samples met GB/T18407-2001 standard and 5 samples failed; the multi-factor integrated index is that 103 samples met NY/P391-2000, in the safe and clean level, 157 samples met GB/T18407-2001, in the safe and clean level, and 5 samples failed, in the alert level but still clean; the single-factor composite index is that 7 townships met NY/P391-2000 standard, 15 townships only met GB/T18407-2001 standard.Ⅱ.The spatial variation trend of five heavy metals in farmland soils, Nong’an County, was that the index values of five heavy metals were mainly high in northwest and southeast. The high value of As concentrated in the central, northwest and southeast; Cd focued on the edge of north, northwest and southwest area; the distribution trend of Cr was interphase from the northeast to southwest; the distribution trend of Pb was interphase from west to east with low and high value; the distribution of Hg was similar to the multi-factor integrated pollution ratings map, which generally concentrated on the west, northwest and southeast.Ⅲ.The regional differentiation rules of heavy metal contamination in Nong’an County farmland soils related natural factors (the soil attribute, hydrological distribution) and human factors (rural townships, transportation, agricultural production, land use types), which were mainly affected by hydrological distribution and the livestock and poultry breeding in the agricultural production. The influence order of all influencing factors was: hydrological distribution>livestock and poultry industry>urban, transportation>plastic sheeting>soil attributes>land use types>fertilizer>pesticide.

Heavy metals in the soil accumulate to a certain amount will lead to soil heavy metal pollution, heavy metals in vegetables to a certain extent would seriously affect their quantity and quality, and affect human health through the food chain. This paper assesses the bioavailability posed by heavy metal content in both soil and tomato in Yangling. The results could be used to guide vegetable safety evaluation.Soil samples of 0~20 cm layer and tomato leaf and fruit were collected from greenhouse in Yangling, carrying on laboratory analysis to study the soil Cd, Cu and Zn form distribution, Cd, Cu and Zn distribution in tomato leaf and fruit, the effect of Cd, Cu and Zn on the rhizospheric microorganism number, preliminary research Cd, Cu and Zn bioavailability. The results were as follows:1. Accumulate coefficient of Cd, Cu and Zn is 2.50, 0.498 and 1.08 respectively, Cd accumulated significant, Zn accumulated small, Cu in soil below background level. There were no obvious interaction between Cd, Cu and Zn.2. To HJ/T 333-2006 for an comprehensive contamination index standard , Cd contamination index raged from 0.7 to 1, were alert levelⅡ, Soil is still clean. Cu, Zn contamination index below 0.7, were security levelⅠ, soil is clean. The study area had not received soil Heavy metals pollution, but Cd had the potential pollution tendency. Need to avoid external Cd into the soil, leading to increased soil pollution. In turn affect food security, cause even greater harm.3. The form alalysis of heavy metals in the greenhouse soil showed that Cd mainly exist in Fe-Mn oxide-bound factions and organically bound form, and Cu mainly exist in residual faction and organic form, with 88.9% in all, Zn mainly exist in Fe-Mn oxide and residual faction, with 75.6% in all. Exchangeable, carbonate, Fe-Mn oxide and residual faction of Cd, Cu and Zn accounted 88.2, 52.4 and 46.1% in the total content separately. Most of heavy metals were in these four forms, which have highly potential risk to the environment, may affect the quality of agricultural products and the agro-ecological environment.4. The content of heavy metals (Cd, Cu and Zn) in the tested tomato fruit were measured all lower to the Chinese Food Hygiene Standard. No sample exceeded the standard, the pass rate of greenhouse tomato fruit content of heavy metals was 100%, meet Chinese Food Hygiene Standards.5. The tomato leaf Cd enrichment capacity is influenced by the available Cd content in soil,and the ability increases along with the available soil Cd content in increase. And the correlations are not obvious between the tomato fruit Cd and each Cd forms in soil. Cu content in soil do not influence the tomato Cu content in leaf and fruit. It has certain relevance between Zn in leaf and fruit of tomato and Zn in soil, and the relevance of different locations not identical. Clearly, the bioavailability was not exactly the same of heavy metals in the soil.6. The proportion of bacteria was more than 90 percent in soil microbial community structure, followed by actinomycetes, at least the number of fungi in the soil. The correlations correlation coefficient between total Cd and the microbes in the soil were negative. And the correlation coefficient between total Zn and microbes were positive. There was no relationship between Cu and the number of soil microorganisms. Heavy metal in soil can affect the soil microbial community structure.

With the increase of water shortage and water pollution in China, the lack of agricultural irrigation water becomes an important factor which hampered China’s agricultural production. The municipal reclaimed water is an important alternative source of water for irrigation. It is an effective method to use city reclaimed water to resolve water shortage in agriculture, but heavy metals in the water may be absorbed by human body through food chain; the excessive amount of them will be harmful, and ultimately affect human health. So it is necessary to use risk assessment technology to evaluate the impacts on human’s health caused by heavy metals in reclaimed water, and then provide the theoretical basis for the using of municipal reclaimed water.This research was based on the demonstrative engineering of municipal reclaimed water for irrigation of farmland in Wuqing District, Tianjin. Corn and wheat were chosen as the experimental crops in the experiment. The impact on soil and crops caused by heavy metals in the reclaimed water irrigation area was analyzed. Monte Carlo method was used to calculate human health risk probability distribution about the heavy metals in crops, then health risk assessment was made, and the key risk factors were decided. The results will contribute to provide referenced basis for the safe use of reclaimed water for irrigation of farmland.The main conclusions will be draw as following:(1) During the experiment, the heavy metals in soil do not show obvious residue; the heavy metal proportion in plant irrigated by municipal reclaimed water is higher than that in control area, but the difference was not significant; seven kinds of heavy metals in the crops were lower than corresponding food hygiene standards and national food hygiene standards, the content of heavy metal is always higher in wheat than in corn, so it is necessary to evaluate the risks.(2)Through the compared among enrichment coefficient of different metals, certain crop has different enrichment capacity of different kinds of metal. Different crops have different enrichment capacity to a certain metal; wheat has stronger enrichment capacity to heavy metal than corn.(3)The health risks analysis of heavy metals in crops:In accordance with international standards, harmful heavy metals could be divided into two kinds: one is chemical carcinogenic metal; the other is non-chemical carcinogenic metal. Lifetime carcinogenic risks of arsenic, cadmium, chromium and lead are less than maximum acceptable level of risk recommended by the International Commission on Radiological Protection (ICRP). Each of the non-carcinogenic heavy metals in the lifetime risks is less than the maximum acceptable level of risk recommended by British Royal Society, the Swedish Environmental Protection Agency and the Netherlands Ministry of Environment. For the individual carcinogenic risk and non-carcinogenic risk of heavy metals, the risk of wheat is higher than that to corn. Based on the assumption that a variety of chemical pollutants have additive risks to human health, the total risk of heavy metal carcinogenic risk and heavy metal non-carcinogenic risk is less than the recommended maximum acceptable level of risk; The total risk of wheat is higher than that of corn, and chromium carcinogenic risk of the total cancer risk is over 70 percent.

With the rapid increase of sewage treatment plants, the disposal and the environmental impact of sewage sludge have been paid more and more attention. Now, land application of sewage sludge has become an important way for sewage sludge disposal duo to its lower cost and higher efficiency. But heavy metals are the key factor that affects the land application of sewage sludge. So we have done some researches on the monitoring of nutrient and heavy metal concentration and the phytoavailability of heavy metal in soil treated of sewage sludge by a pot experiment. Also the impacts of soil environmental and heavy metal contents in wheat by land applications of sewage sludge were evaluated to assess the agronomic benefits and environmental impacts of sewage sludge application to soils. The main results obtained were as the following:1. The content of organic matters, nitrogen, phosphorus and potassium in sewage sludge from Beijing is 35.5%, 2.7%, 3.8% and 0.2%, respectively. Except potassium, those are higher than organic fertilizer as pig manure and chicken manure.2. The mean concentrations of Zn, Cu, Cr, Pb, Ni, As and Hg in sewage sludge from sludge disposal plant are 1665 mg/kg, 238 mg/kg, 98 mg/kg, 79 mg/kg, 46 mg/kg, 20 mg/kg and 13 mg/kg, respectively, and are 821 mg/kg, 149 mg/kg, 31 mg/kg, 35 mg/kg, 40 mg/kg, 21 mg/kg, 14 mg/kg respectively from sewage treatment plant. Compared with the heavy metal limits of sewage sludge for land application in china, Zn, Cu, Cr, Pb, Ni and As concentrations in sewage sludge are lower than the limits value, and Hg concentrations in sewage sludge is overran the limits value for pH

As an advanced absorbent, biological absorbent which has been proved to be engineering feasible and economical beneficial, has an extensive vista of research and large advantages in the treatment of wastewater that contains heavy metals.An SBR reactor was used to discuss the feasibility and utility of heavy metal biosorption by activated sludge in the treatment of desulfurization wastewater which contains traces of heavy matals. The biosorption capacities of activated sludge towards Cr（Ⅲ）, Cd（Ⅱ） and Pb（Ⅱ） in the desulfurization wastewater were observed. In this study, the influences of different hydraulic retention times（HRT）, MLSS, initial concentrations of heavy metals and pH on the heavy metal biosorption process were assessed and an optimum operation condition of heavy metal biosorption by activated sludge process were obtained. The research results are as follows:（1） HRT=16h is an optimum operation time for sufficient biosorption and at the same time non-desorption towards heavy metals, the biosorption efficiency is at its best with a value of 71 %.（2） When the concentrations of activated sludge （MLSS） are 5000～5500 mg/L, the metal biosorption capacity of activated sludge reaches a highest value, with COD removal rate of 88.7% and Cr （Ⅲ） removal rate of 70%.（3） when the initial Cr（Ⅲ） concentrations are 1～600μg /L, the capacity and efficiency of Cr biosorption are increasing constantly with the increase of initial Cr（Ⅲ） concentrations and reaches an extreme of 85% by biosorption efficiency at the Cr（Ⅲ） concentration of 600μg /L. However, the biosorption efficiency of Cr begins to drop when the initial Cr（Ⅲ） concentrations are 600～1000μg/L while the biosorption capacity remains to increase. The biosorption efficiency lowered to 78% at the initial Cr（Ⅲ） concentration of 980μg/L.（4） pH values influence heavy metal biosorption by activated sludge most. When pH<4, the out-lowered pH leads to the death of activated sludge; when pH>7, biosorpation capacity is affected by reducing the adsorption points and weakening the adsorption capability of points.In the experiment, pH=6 is optimal for heavy metal biosorption of activated sludge with the biosorption capacity and biosorption efficiency of 12.82μg/g and 72.3 %, respectively.（5） Heavy metals compete for binding sites of activated sludge in case that several metals are contained. This competition leads to the inhibition of heavy metal biosorption, and the biosorption capacity of target ions will be lowered. The Cr（Ⅲ） removal rates are lowered to 44% and 63%, compare to the condition of only existing Cr（Ⅲ）,which are fell by 41% and 22%, when Pb （Ⅱ） and Cd （Ⅱ） are added to the system, respectively. The existence of Pb （Ⅱ） and Cd （Ⅱ） affects the biosorption of activated sludge towards Cr（Ⅲ）.（6） Simulate the heavy metal biosorption of activated sludge using Langmuir and Freundlich adsorption models. It’s obtained that Cd（Ⅱ） and Pb（Ⅱ） biosorptions fit Langmuir adsorption model and Cr（Ⅲ） biosorption fits Freundlich adsorption model. The metal-sludge affinity order is obtained as follow: Pb（Ⅱ）>Cd（Ⅱ）>Cr（Ⅱ）.（7） An SBR activated sludge process is practical for heavy metal treatment in full-scale desulfurization wastewater to the discharge standard abroad. In this study, the main heavy metals in the desulfurization wastewater such as Cd²⁺, Cr³⁺, Hg²⁺, Pb²⁺ and Zn²⁺ are treated to 21μg /L, 17μg /L, 8μg /L, 13μg /L and 215μg /L, resbectively, which are all lower than the discharge standard abroad.（8） Through the research on microorganism of activated sludge, founding that the Epistylis Vrceolata played a major role in the absorption of heavy metal by activated sludge. At the same time there are a lot of Protozoans and Epigenetic animals exist in the activated sludge, it will reduce the amount of sludge theoretically and able to achieve the objective of reducing.

As an advanced absorbent, biological absorbent which has been proved to be engineering feasible and economical beneficial, has an extensive vista of research and large advantages in the treatment of wastewater that contains heavy metals.An SBR reactor was used to discuss the feasibility and utility of heavy metal biosorption by activated sludge in the treatment of desulfurization wastewater which contains traces of heavy matals. The biosorption capacities of activated sludge towards Cr（Ⅲ）, Cd（Ⅱ） and Pb（Ⅱ） in the desulfurization wastewater were observed. In this study, the influences of different hydraulic retention times（HRT）, MLSS, initial concentrations of heavy metals and pH on the heavy metal biosorption process were assessed and an optimum operation condition of heavy metal biosorption by activated sludge process were obtained. The research results are as follows:（1） HRT=16h is an optimum operation time for sufficient biosorption and at the same time non-desorption towards heavy metals, the biosorption efficiency is at its best with a value of 71 %.（2） When the concentrations of activated sludge （MLSS） are 5000～5500 mg/L, the metal biosorption capacity of activated sludge reaches a highest value, with COD removal rate of 88.7% and Cr （Ⅲ） removal rate of 70%.（3） when the initial Cr（Ⅲ） concentrations are 1～600μg /L, the capacity and efficiency of Cr biosorption are increasing constantly with the increase of initial Cr（Ⅲ） concentrations and reaches an extreme of 85% by biosorption efficiency at the Cr（Ⅲ） concentration of 600μg /L. However, the biosorption efficiency of Cr begins to drop when the initial Cr（Ⅲ） concentrations are 600～1000μg/L while the biosorption capacity remains to increase. The biosorption efficiency lowered to 78% at the initial Cr（Ⅲ） concentration of 980μg/L.（4） pH values influence heavy metal biosorption by activated sludge most. When pH<4, the out-lowered pH leads to the death of activated sludge; when pH>7, biosorpation capacity is affected by reducing the adsorption points and weakening the adsorption capability of points.In the experiment, pH=6 is optimal for heavy metal biosorption of activated sludge with the biosorption capacity and biosorption efficiency of 12.82μg/g and 72.3 %, respectively.（5） Heavy metals compete for binding sites of activated sludge in case that several metals are contained. This competition leads to the inhibition of heavy metal biosorption, and the biosorption capacity of target ions will be lowered. The Cr（Ⅲ） removal rates are lowered to 44% and 63%, compare to the condition of only existing Cr（Ⅲ）,which are fell by 41% and 22%, when Pb （Ⅱ） and Cd （Ⅱ） are added to the system, respectively. The existence of Pb （Ⅱ） and Cd （Ⅱ） affects the biosorption of activated sludge towards Cr（Ⅲ）.（6） Simulate the heavy metal biosorption of activated sludge using Langmuir and Freundlich adsorption models. It’s obtained that Cd（Ⅱ） and Pb（Ⅱ） biosorptions fit Langmuir adsorption model and Cr（Ⅲ） biosorption fits Freundlich adsorption model. The metal-sludge affinity order is obtained as follow: Pb（Ⅱ）>Cd（Ⅱ）>Cr（Ⅱ）.（7） An SBR activated sludge process is practical for heavy metal treatment in full-scale desulfurization wastewater to the discharge standard abroad. In this study, the main heavy metals in the desulfurization wastewater such as Cd²⁺, Cr³⁺, Hg²⁺, Pb²⁺ and Zn²⁺ are treated to 21μg /L, 17μg /L, 8μg /L, 13μg /L and 215μg /L, resbectively, which are all lower than the discharge standard abroad.（8） Through the research on microorganism of activated sludge, founding that the Epistylis Vrceolata played a major role in the absorption of heavy metal by activated sludge. At the same time there are a lot of Protozoans and Epigenetic animals exist in the activated sludge, it will reduce the amount of sludge theoretically and able to achieve the objective of reducing.

The heavy metal cadmium （Cd）, lead（Pb）, copper（Cu）, chromium（Cr） is a kind of environmental pollutant with stronger toxicity in agricultural ecosystems,which greatly influences the growth and development, physiological and genetic metabolic of the plant. In this paper Hordeum vulgare is selected as the material in the experiment. Indoor foster method is applied to study the effects of Cd²⁺, Pb²⁺, Cu²⁺, Cr⁶⁺ in different concentrations (5 mg·L^-1, 10 mg·L^-1, 20 mg·L^-1, 40mg·L^-1, 80 mg·L^-1) on Hordeum vulgare from the aspects of accumulation and migration, some physiological and biochemical indicators of molecular and genomic DNA damage. The results are as follows:The dynamic changing rule of Cd²⁺,Pb²⁺,Cu²⁺,Cr⁶⁺ of different concentrations on absorbing,accumulating and distributing in barley seedling. The results shows that with the increasing of the treatment concentrations, the content of four heavy metals in barley seedling increased; with the time increasing, the content of heavy metal in roots of the barley seedling presents the trend of reducing after rising, while the content of heavy mental in the stems and leaves is like this: first rising, then falling, then rising. By comparing the content of four heavy metals in the barley seedling its accumulating order in the roots is Pb²⁺>Cu²⁺>Cd²⁺>Cr⁶⁺; in the stems and leaves is Cu²⁺>Cd²⁺>Pb²⁺>Cr⁶⁺ ;the ability of transferring from roots to stems and leaves is Cu²⁺>Cd²⁺>Cr⁶⁺>Pb²⁺. The content of heavy metals in the roots was more than in the stems and leaves.Effects of Cd²⁺,Pb²⁺,Cu²⁺,Cr⁶⁺ in different concentrations on the soluble sugar, soluble protein and ATPase activity in leaves of barley seedling are studied. The results indicated that under the poisoning of Cd ,Pb ,Cu ,Cr , （1） the soluble sugar content in barley seedling leaves gradually reduces with the concentration and processing time increasing. （2） the soluble protein content of barley seedling leaves rapidly declining with Cd²⁺,Cr⁶⁺, Pb²⁺concentration increased, whereas with Cu²⁺ dealing with the barley, the formation of the soluble protein content in the leaves is promoted under the low density, and is inhibited and formatted under the high density. （3） Cd²⁺,Cr⁶⁺ on barley seedlings can inhibit the ATPase activity, for with the performance of the Cd²⁺,Cr⁶⁺ concentration increasing and processing time extending, ATPase activity of barley seedling leaves gradually declines. Cu ,Pb on the activity of ATPase presents the trend of promoting in the low concentrations and inhibiting in the high concentration, for the performance of the activity of the ATPase increases first, then declines with the Cu²⁺,Pb²⁺ concentration. The influence of Cd , Pb , Cu , Cr stress upon the content of DNA of the barely seedling and its damaging effect of the DNA in the root of seedlings. The results indicated that （1） the content of DNA in the roots and leaves of barely seedling decreases with the treatment of Cd²⁺,Pb²⁺,Cu²⁺,Cr⁶⁺ and the content of DNA in the roots decreases greatly than that in the leaves. （2） Different kind of heavy mental treatment causes different DNA damaging form to the barley seedling. With the treatment of Cu the DNA hyperchromic effects higher than the compared ones shows that Cu²⁺ treatment in the trialed intensity causes DNA fragmentation of the barley seedlings. With the treatment of Pb²⁺ the DNA hyperchromic effects lower than the compared ones shows that Pb²⁺ treatment in the trialed intensity causes DNA cross-linking of the barley seedlings. The treatment of Cd²⁺,Cr⁶⁺ the DNA hyperchromic effects has the trend of increasing first, then decreasing, which shows that low concentration of Cd²⁺,Cr⁶⁺ causes DNA fragmentation the barley seedlings, the high concentration of them leads to DNA cross-linking. （3） The changes occurring in random amplified polymorphic DNA（RAPD） profiles of barley seedling following Cr⁶⁺ treatment includes appearance of new bands and loss of normal bands and variation in band intensity compared with the normal seedlings. These changes of DNA polymorphisms are positive correlation with the Cr⁶⁺ concentration.